BMRB Entry 6190

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6190
MolProbity Validation Chart

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Title:
Solution Structure of apoCox11
Deposition date:
2004-04-22
Original release date:
2004-06-30
Authors:
Banci, L.; Bertini, I.; Cantini, F.; Ciofi-Baffoni, S.; Gonnelli, L.; Mangani, S.
Citation:

Citation: Banci, L.; Bertini, I.; Cantini, F.; Ciofi-Baffoni, S.; Gonnelli, L.; Mangani, S.. "Solution structure of Cox11: A novel type of beta-immunoglobulin-like fold involved in CuB site formation of cytochrome c oxidase"  J. Biol. Chem. ., .-. (2004).
PubMed: 15181013

Assembly members:

Assembly members:
Cytochrome C oxidase assembly protein ctaG, polymer, 164 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Sinorhizobium meliloti   Taxonomy ID: 382   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Sinorhizobium melilot

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET21A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cytochrome C oxidase assembly protein ctaG: MVPLYDMFCRVTGYNGTTQR VEQASDLILDEKIKVTFDAN VAAGLPWEFVPVQRDIDVRI GETVQIMYRAKNLASTPTTG QATFNVTPMAAGAYFNKVQC FCFTETTLEPGEEMEMPVVF FVDPEIVKPVETQGIKTLTL SYTFYPREPSKPVAQVKAKA ENKL

Data sets:
Data typeCount
1H chemical shifts878
13C chemical shifts330
15N chemical shifts136

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cytochrome C oxidase assembly protein ctaG1

Entities:

Entity 1, Cytochrome C oxidase assembly protein ctaG 164 residues - Formula weight is not available

1   METVALPROLEUTYRASPMETPHECYSARG
2   VALTHRGLYTYRASNGLYTHRTHRGLNARG
3   VALGLUGLNALASERASPLEUILELEUASP
4   GLULYSILELYSVALTHRPHEASPALAASN
5   VALALAALAGLYLEUPROTRPGLUPHEVAL
6   PROVALGLNARGASPILEASPVALARGILE
7   GLYGLUTHRVALGLNILEMETTYRARGALA
8   LYSASNLEUALASERTHRPROTHRTHRGLY
9   GLNALATHRPHEASNVALTHRPROMETALA
10   ALAGLYALATYRPHEASNLYSVALGLNCYS
11   PHECYSPHETHRGLUTHRTHRLEUGLUPRO
12   GLYGLUGLUMETGLUMETPROVALVALPHE
13   PHEVALASPPROGLUILEVALLYSPROVAL
14   GLUTHRGLNGLYILELYSTHRLEUTHRLEU
15   SERTYRTHRPHETYRPROARGGLUPROSER
16   LYSPROVALALAGLNVALLYSALALYSALA
17   GLUASNLYSLEU

Samples:

sample_1: Cytochrome C oxidase assembly protein ctaG, [U-15N], 1.5 mM; DTT 5 mM; phosphate buffer 20 mM; H2O 90%; D2O 10%

sample_2: Cytochrome C oxidase assembly protein ctaG, [U-95% 13C; U-98% 15N], 1 mM; DTT 5 mM; phosphate buffer 20 mM; H2O 90%; D2O 10%

sample_3: Cytochrome C oxidase assembly protein ctaG, [U-95% 13C; U-98% 15N], 2 mM; DTT 5 mM; phosphate buffer 20 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 7; temperature: 298 K; ionic strength: 20 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
2D TOCSYnot availablenot availablesample_cond_1
3D 15N-separated NOESYnot availablenot availablesample_cond_1
HNHAnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
13C (H)CCH-TOCSYnot availablenot availablesample_cond_1
CBCA(CO)NHnot availablenot availablesample_cond_1
CBCANHnot availablenot availablesample_cond_1
HNCOnot availablenot availablesample_cond_1
HN(CA)COnot availablenot availablesample_cond_1

Software:

XWINNMR - collection

XEASY v1.3 - data analysis

DYANA v1.5 - structure solution

CYANA v1.0 - structure solution

AMBER v5.0 - refinement

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
EMBL CAC45480 CCM66608 CDH84806
GB AEG03463 AEG52377 AEH77844 AGA05872 AGG73486
REF NP_385014 WP_003532324 WP_010968908 WP_015445564 WP_018094447
SP Q92RG6
AlphaFold Q92RG6 Q92RG6

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks