BMRB Entry 6186

Title:
Structure of DNA sequence d-TGATCA by two-dimensional nuclear magnetic resonance spec and restrained molecular dynamics
Deposition date:
2004-04-22
Original release date:
2004-11-08
Authors:
Barthwal, R.; Awasthi, P.; Narang, M.; Sharma, U.; Srivastava, N.
Citation:

Citation: Barthwal, R.; Awasthi, P.; Monica, .; Kaur, M.; Sharma, U.; Srivastava, N.; Barthwal, S.; Govil, G.. "Structure of DNA sequence d-TGATCA by two-dimensional nuclear magnetic resonance spectroscopy and restrained molecular dynamics"  J. Struct. Biol. 148, 34-50 (2004).
PubMed: 15363786

Assembly members:

Assembly members:
TGATCA, polymer, 6 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TGATCA: TGATCA

Data sets:
Data typeCount
1H chemical shifts47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15'-D(P*TP*GP*AP*TP*CP*A)-3', chain 11
25'-D(P*TP*GP*AP*TP*CP*A)-3', chain 21

Entities:

Entity 1, 5'-D(P*TP*GP*AP*TP*CP*A)-3', chain 1 6 residues - Formula weight is not available

1   DTDGDADTDCDA

Samples:

sample_1: TGATCA 7.36 mM; phosphate buffer 20 mM; NaCl 15 mM; D2O 100%

sample_2: TGATCA 9.14 mM; phosphate buffer 20 mM; NaCl 15 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 35 mM; pH: 7.0; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablesample_cond_1
DQF-COSYnot availablenot availablesample_cond_1

Software:

DISCOVER v97 - refinement

NMR spectrometers:

  • Bruker AM 500 MHz