BMRB Entry 6183

Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for cAMP-dependent protein kinase
Published: 2005-04-20

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6183

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for cAMP-dependent protein kinase

Deposition date:

2004-04-20

Original release date:

2005-04-20

Authors:

Thomas, Langer; Martin, Vogtherr; Bettina, Elshorst; Marco, Betz; Ulrich, Schieborr; Krishna, Saxena; Harald, Schwalbe

Citation:

Citation: Langer, Thomas; Vogtherr, Martin; Elshorst, Bettina; Betz, Marco; Schieborr, Ulrich; Saxena, Krishna; Schwalbe, Harald. "NMR backbone assignment of a protein kinase catalytic domain by a combination of several approaches: Application to the catalytic subunit of cAMP-dependent protein kinase" Chembiochem 5, 1508-1516 (2004).
PubMed: 15481030

Assembly members:

Assembly members:
protein kinase catalytic domain, polymer, 353 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Chinese hamster Taxonomy ID: 10029 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Cricetulus griseus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
protein kinase catalytic domain: GHMGNAAAAKKGSEQESVKE FLAKAKEEFLKKWESPSQNT AQLDHFDRIKTLGTGSFGRV MLVKHKETGNHYAMKILDKQ KVVKLKQIEHTLNEKRILQA VNFPFLVKLEFSFKDNSNLY MVMEYVPGGEMFSHLRRIGR FSEPHARFYAAQIVLTFEYL HSLDLIYRDLKPENLLIDQQ GYIQVTDFGFAKRVKGRTWT LCGTPEYLAPEIILSKGYNK AVDWWALGVLIYEMAAGYPP FFADQPIQIYEKIVSGKVRF PSHFSSDLKDLLRNLLQVDL TKRFGNLKNGVNDIKNHKWF ATTDWIAIYQRKVEAPFIPK FKGPGDTSNFDDYEEEEIRV SINEKCGKEFTEF

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	191
13C chemical shifts	579
15N chemical shifts	191

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cAMP dependent protein kinase catalytic domain	1

Entities:

Entity 1, cAMP dependent protein kinase catalytic domain 353 residues - Formula weight is not available

1

GLY

HIS

MET

GLY

ASN

ALA

LYS

2

LYS

GLY

SER

GLU

GLN

GLU

SER

VAL

LYS

GLU

3

PHE

LEU

ALA

LYS

ALA

LYS

GLU

PHE

LEU

4

LYS

TRP

GLU

SER

PRO

SER

GLN

ASN

THR

5

ALA

GLN

LEU

ASP

HIS

PHE

ASP

ARG

ILE

LYS

6

THR

LEU

GLY

THR

GLY

SER

PHE

GLY

ARG

VAL

7

MET

LEU

VAL

LYS

HIS

LYS

GLU

THR

GLY

ASN

8

HIS

TYR

ALA

MET

LYS

ILE

LEU

ASP

LYS

GLN

9

LYS

VAL

LYS

LEU

LYS

GLN

ILE

GLU

HIS

10

THR

LEU

ASN

GLU

LYS

ARG

ILE

LEU

GLN

ALA

11

VAL

ASN

PHE

PRO

PHE

LEU

VAL

LYS

LEU

GLU

12

PHE

SER

PHE

LYS

ASP

ASN

SER

ASN

LEU

TYR

13

MET

VAL

MET

GLU

TYR

VAL

PRO

GLY

GLU

14

MET

PHE

SER

HIS

LEU

ARG

ILE

GLY

ARG

15

PHE

SER

GLU

PRO

HIS

ALA

ARG

PHE

TYR

ALA

16

ALA

GLN

ILE

VAL

LEU

THR

PHE

GLU

TYR

LEU

17

HIS

SER

LEU

ASP

LEU

ILE

TYR

ARG

ASP

LEU

18

LYS

PRO

GLU

ASN

LEU

ILE

ASP

GLN

19

GLY

TYR

ILE

GLN

VAL

THR

ASP

PHE

GLY

PHE

20

ALA

LYS

ARG

VAL

LYS

GLY

ARG

THR

TRP

THR

21

LEU

CYS

GLY

THR

PRO

GLU

TYR

LEU

ALA

PRO

22

GLU

ILE

LEU

SER

LYS

GLY

TYR

ASN

LYS

23

ALA

VAL

ASP

TRP

ALA

LEU

GLY

VAL

LEU

24

ILE

TYR

GLU

MET

ALA

GLY

TYR

PRO

25

PHE

ALA

ASP

GLN

PRO

ILE

GLN

ILE

TYR

26

GLU

LYS

ILE

VAL

SER

GLY

LYS

VAL

ARG

PHE

27

PRO

SER

HIS

PHE

SER

ASP

LEU

LYS

ASP

28

LEU

ARG

ASN

LEU

GLN

VAL

ASP

LEU

29

THR

LYS

ARG

PHE

GLY

ASN

LEU

LYS

ASN

GLY

30

VAL

ASN

ASP

ILE

LYS

ASN

HIS

LYS

TRP

PHE

31

ALA

THR

ASP

TRP

ILE

ALA

ILE

TYR

GLN

32

ARG

LYS

VAL

GLU

ALA

PRO

PHE

ILE

PRO

LYS

33

PHE

LYS

GLY

PRO

GLY

ASP

THR

SER

ASN

PHE

34

ASP

TYR

GLU

ILE

ARG

VAL

35

SER

ILE

ASN

GLU

LYS

CYS

GLY

LYS

GLU

PHE

36

THR

GLU

PHE

Samples:

sample_1: protein kinase catalytic domain, [U-100% 13C; U-100% 15N; U-100% 2H], 0.4 – 0.6 mM; NaCl 300 mM; Hepes 50 mM; Dithiotheithrol 5 mM

Ex-cond_1: pH: 6.0; temperature: 298 K; ionic strength: 0.3 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N TOCSY	sample_1	not available	Ex-cond_1
HNCO	sample_1	not available	Ex-cond_1
HN(CA)CO	sample_1	not available	Ex-cond_1
HNCACB	sample_1	not available	Ex-cond_1
HN(CO)CACB	sample_1	not available	Ex-cond_1
HNCA	sample_1	not available	Ex-cond_1

Software:

XEASY v1.3.14 - assignment

NMR spectrometers:

Bruker DRX 800 MHz

PDB	1APM 1ATP 1BKX 1BX6 1CTP 1FMO 1J3H 1JBP 1JLU 1L3R 1RDQ 1RE8 1REJ 1REK 1SVH 1SYK 1XH5 1XH6 1XH8 2C1A 2C1B 2CPK 2ERZ 2F7E 2F7X 2F7Z 2GFC 2GU8 2OH0 2OJF 2QCS 2QUR 2QVS 2UZT 2UZU 2UZV 2UZW 2VO7 3AG9 3AGL 3AGM 3FHI 3FJQ 3IDB 3IDC 3J4Q 3J4R 3L9L 3MVJ 3NX8 3O7L 3OOG 3OVV 3OW3 3OWP 3OXT 3P0M 3POO 3PVB 3QAL 3QAM 3TNP 3TNQ 3VQH 3ZO4 4DFX 4DFY 4DFZ 4DG0 4DG2 4DG3 4DH1 4DH3 4DH5 4DH7 4DH8 4DIN 4HPT 4HPU 4IAC 4IAD 4IAF 4IAI 4IAK 4IAY 4IAZ 4IB0 4IB1 4IB3 4IE9 4IJ9 4NTS 4NTT 4O21 4O22 4WIH
DBJ	BAF82836 BAH13444 BAI46794
EMBL	CAA30597 CAA41052 CAA47627
GB	AAA37010 AAA39936 AAA39937 AAA60094 AAF76423
REF	NP_001003032 NP_001009234 NP_001094392 NP_001264827 NP_001291278
SP	P00517 P05132 P17612 P25321 P27791
TPG	DAA28080
AlphaFold	P00517 P05132 P17612 P25321 P27791