BMRB Entry 6179
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6179
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tidow, H.; Lauber, T.; Vitzithum, Klaus; Sommerhoff, C.; Roesch, Paul; Marx, Ute. "The Solution structure of a chimeric LEKTI domain reveals a chameleon sequence" Biochemistry 43, 11238-11247 (2004).
PubMed: 15366933
Assembly members:
LEKTI Domain 15 short, polymer, 61 residues, 6922.9 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: T7-Expressionsvector
Entity Sequences (FASTA):
LEKTI Domain 15 short: GPDSEMCKDYRVLPRIGYLC
PKDLKPVCGDDGQTYNNPCM
LCHENLIRQTNTHIRSTGKC
E
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 51 |
| 1H chemical shifts | 358 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LEKTI Domain 15 short | 1 |
Entities:
Entity 1, LEKTI Domain 15 short 61 residues - 6922.9 Da.
| 1 | GLY | PRO | ASP | SER | GLU | MET | CYS | LYS | ASP | TYR | ||||
| 2 | ARG | VAL | LEU | PRO | ARG | ILE | GLY | TYR | LEU | CYS | ||||
| 3 | PRO | LYS | ASP | LEU | LYS | PRO | VAL | CYS | GLY | ASP | ||||
| 4 | ASP | GLY | GLN | THR | TYR | ASN | ASN | PRO | CYS | MET | ||||
| 5 | LEU | CYS | HIS | GLU | ASN | LEU | ILE | ARG | GLN | THR | ||||
| 6 | ASN | THR | HIS | ILE | ARG | SER | THR | GLY | LYS | CYS | ||||
| 7 | GLU |
Samples:
sample_1: LEKTI Domain 15 short, [U-15N], 0.7 mM
sample_2: LEKTI Domain 15 short 1.6 mM
sample_cond_1: ionic strength: 20 mM; pH: 6.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D-TOCSY | not available | not available | sample_cond_1 |
| 2D-COSY | not available | not available | sample_cond_1 |
| 2D-NOESY | not available | not available | sample_cond_1 |
| 1H,15N-HSQC | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| 3D-1H,1H,15N-TOCSY-HSQC | not available | not available | sample_cond_1 |
| 3D-1H,1H,15N-NOESY-HSQC | not available | not available | sample_cond_1 |
| 3D-1H,15N,15N-HMQC-NOESY-HSQC | not available | not available | sample_cond_1 |
Software:
NMRview v5.0.4 -
NDEE, Spinup Inc. -
NMR spectrometers:
- Bruker DRX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks