BMRB Entry 6178

Name: N-terminal Domain of the Polypyrimidine Tract Binding Protein Contains a New RRM Variant That Contributes to High-Affinity RNA Binding
Published: 2004-11-08

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PDB ID: 1sjq
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6178
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

N-terminal Domain of the Polypyrimidine Tract Binding Protein Contains a New RRM Variant That Contributes to High-Affinity RNA Binding

Deposition date:

2004-04-15

Original release date:

2004-11-08

Authors:

Simpson, P.; Monie, T.; Szendroi, A.; Davydova, N.; Tyzack, J.; Conte, M.; Read, C.; Cary, P.; Svergun, D.; Konarev, P.; Petoukhov, M.; Curry, S.; Matthews, S.

Citation:

Citation: Simpson, P.; Monie, T.; Szendroi, A.; Davydova, N.; Tyzack, J.; Conte, M.; Read, C.; Cary, P.; Svergun, D.; Konarev, P.; Curry, S.; Matthews, S.. "Structure and RNA interactions of the N-terminal RRM domains of PTB" Structure (Camb) 12, 1631-1643 (2004).
PubMed: 15341728

Assembly members:

Assembly members:
polypyrimidine tract binding protein 1 domain 1, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
polypyrimidine tract binding protein 1 domain 1: MRGSHHHHHHGSGVPSRVIH IRKLPIDVTEGEVISLGLPF GKVTNLLMLKGKNQAFIEMN TEEAANTMVNYYTSVTPVLR GQPIYIQFSNHKELKTDSSP NQARA

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	653
13C chemical shifts	411
15N chemical shifts	95

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PTB1-1 monomer	1

Entities:

Entity 1, PTB1-1 monomer 105 residues - Formula weight is not available

1

MET

ARG

GLY

SER

HIS

2

GLY

SER

GLY

VAL

PRO

SER

ARG

VAL

ILE

HIS

3

ILE

ARG

LYS

LEU

PRO

ILE

ASP

VAL

THR

GLU

4

GLY

GLU

VAL

ILE

SER

LEU

GLY

LEU

PRO

PHE

5

GLY

LYS

VAL

THR

ASN

LEU

MET

LEU

LYS

6

GLY

LYS

ASN

GLN

ALA

PHE

ILE

GLU

MET

ASN

7

THR

GLU

ALA

ASN

THR

MET

VAL

ASN

8

TYR

THR

SER

VAL

THR

PRO

VAL

LEU

ARG

9

GLY

GLN

PRO

ILE

TYR

ILE

GLN

PHE

SER

ASN

10

HIS

LYS

GLU

LEU

LYS

THR

ASP

SER

PRO

11

ASN

GLN

ALA

ARG

ALA

Samples:

sample_1: polypyrimidine tract binding protein 1 domain 1 1 mM; Na phosphate 50 mM; NaCl 100 mM; sodium azide 2 mM; DTT 10 mM

sample_cond_1: pH: 6.5; temperature: 303 K; ionic strength: 0.3 M; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
HSQC	sample_1	not available	sample_cond_1
HNCACB	sample_1	not available	sample_cond_1
CBCA(CO)NH	sample_1	not available	sample_cond_1
HNCO	sample_1	not available	sample_cond_1
HN(CA)CO	sample_1	not available	sample_cond_1
HCCH-TOCSY	sample_1	not available	sample_cond_1
3D 13C-separated NOESY	sample_1	not available	sample_cond_1
3D 15N-separated NOESY	sample_1	not available	sample_cond_1

Software:

XWINNMR v3.1 - collection, processing

AURELIA v2.8.11 - data analysis

NMRPipe v1 - processing

NMRView v4.1.3 - data analysis

X-PLOR v3.851 - structure solution, refinement

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 500 MHz

PDB	1SJQ
DBJ	BAC29560 BAD92147 BAI46909
EMBL	CAA43056 CAA43973 CAA46443 CAA46444 CAA47386
GB	AAC99798 AAH02397 AAH04383 AAH13694 AAP35465
REF	NP_001269942 NP_002810 NP_114367 NP_114368 XP_001092088
SP	P26599
AlphaFold	P26599