BMRB Entry 6161

Name: Full 1H, 13C and 15N Chemical Shift Assignments for the GI Domain of the yeast Linker Histone, Hho1p
Published: 2005-02-07

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PDB ID: 1ust
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6161
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Full 1H, 13C and 15N Chemical Shift Assignments for the GI Domain of the yeast Linker Histone, Hho1p

Deposition date:

2004-04-01

Original release date:

2005-02-07

Authors:

Coles, Patrick; Thomas, Jean; Stott, Katherine; Stevens, Timothy; Ali, Tariq

Citation:

Citation: Ali, Tariq; Coles, Patrick; Stevens, Timothy; Stott, Katherine; Thomas, Jean. "Two homologous domains of similar structure but different stability in the yeast linker histone, Hho1p" J. Mol. Biol. 338, 139-148 (2004).
PubMed: 15050829

Assembly members:

Assembly members:
Yeast linker histone globular domain I, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yeast linker histone globular domain I: KKEEASSKSYRELIIEGLTA LKERKGSSRPALKKFIKENY PIVGSASNFDLYFNNAIKKG VEAGDFEQPKGPAGAVKLAK KKSPEVKKEKEVS

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	334
15N chemical shifts	94
1H chemical shifts	662

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hho1p GI	1

Entities:

Entity 1, Hho1p GI 93 residues - Formula weight is not available

1

LYS

GLU

ALA

SER

LYS

SER

TYR

2

ARG

GLU

LEU

ILE

GLU

GLY

LEU

THR

ALA

3

LEU

LYS

GLU

ARG

LYS

GLY

SER

ARG

PRO

4

ALA

LEU

LYS

PHE

ILE

LYS

GLU

ASN

TYR

5

PRO

ILE

VAL

GLY

SER

ALA

SER

ASN

PHE

ASP

6

LEU

TYR

PHE

ASN

ALA

ILE

LYS

GLY

7

VAL

GLU

ALA

GLY

ASP

PHE

GLU

GLN

PRO

LYS

8

GLY

PRO

ALA

GLY

ALA

VAL

LYS

LEU

ALA

LYS

9

LYS

SER

PRO

GLU

VAL

LYS

GLU

LYS

10

GLU

VAL

SER

Samples:

sample_1: Yeast linker histone globular domain I, [U-95% 13C; U-95% 15N], 2.7 mM; sodium phosphate 100 mM; DSS 10 mM; D2O 99.8%

Ex-cond_1: ionic strength: 0.10 M; pH: 7.0; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY-15N-HSQC	sample_1	not available	Ex-cond_1
15N-HNHA	sample_1	not available	Ex-cond_1
15N-HSQC-TOCSY	sample_1	not available	Ex-cond_1
HNCA	sample_1	not available	Ex-cond_1
HNCACB	sample_1	not available	Ex-cond_1
HN(CO)CA	sample_1	not available	Ex-cond_1
CBCA(CO)NH	sample_1	not available	Ex-cond_1
(H)CC(CO)NH-TOCSY	sample_1	not available	Ex-cond_1
HC(C)H-TOCSY	sample_1	not available	Ex-cond_1
NOESY-13C-HSQC	sample_1	not available	Ex-cond_1

Software:

AZARA v2.7 - Spectrum processing

ANSIG v3.3 - Assignment

NMR spectrometers:

Bruker DRX 500 MHz

PDB	1UHM 1UST
GB	EGA60263 EGA76627 EGA84535