BMRB Entry 6161

Title:
Full 1H, 13C and 15N Chemical Shift Assignments for the GI Domain of the yeast Linker Histone, Hho1p   PubMed: 15050829
Deposition date:
2004-04-01
Original release date:
2005-02-07
Authors:
Coles, Patrick; Thomas, Jean; Stott, Katherine; Stevens, Timothy; Ali, Tariq
Citation:

Citation: Ali, Tariq; Coles, Patrick; Stevens, Timothy; Stott, Katherine; Thomas, Jean. "Two homologous domains of similar structure but different stability in the yeast linker histone, Hho1p"  J. Mol. Biol. 338, 139-148 (2004).

Assembly members:

Assembly members:
Yeast linker histone globular domain I, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Yeast linker histone globular domain I: KKEEASSKSYRELIIEGLTA LKERKGSSRPALKKFIKENY PIVGSASNFDLYFNNAIKKG VEAGDFEQPKGPAGAVKLAK KKSPEVKKEKEVS

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts94
1H chemical shifts662

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hho1p GI1

Entities:

Entity 1, Hho1p GI 93 residues - Formula weight is not available

1   LYSLYSGLUGLUALASERSERLYSSERTYR
2   ARGGLULEUILEILEGLUGLYLEUTHRALA
3   LEULYSGLUARGLYSGLYSERSERARGPRO
4   ALALEULYSLYSPHEILELYSGLUASNTYR
5   PROILEVALGLYSERALASERASNPHEASP
6   LEUTYRPHEASNASNALAILELYSLYSGLY
7   VALGLUALAGLYASPPHEGLUGLNPROLYS
8   GLYPROALAGLYALAVALLYSLEUALALYS
9   LYSLYSSERPROGLUVALLYSLYSGLULYS
10   GLUVALSER

Samples:

sample_1: Yeast linker histone globular domain I, [U-95% 13C; U-95% 15N], 2.7 mM; sodium phosphate 100 mM; DSS 10 mM; D2O 99.8%

Ex-cond_1: ionic strength: 0.10 M; pH: 7.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
NOESY-15N-HSQCsample_1not availableEx-cond_1
15N-HNHAsample_1not availableEx-cond_1
15N-HSQC-TOCSYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
CBCA(CO)NHsample_1not availableEx-cond_1
(H)CC(CO)NH-TOCSYsample_1not availableEx-cond_1
HC(C)H-TOCSYsample_1not availableEx-cond_1
NOESY-13C-HSQCsample_1not availableEx-cond_1

Software:

AZARA v2.7 - Spectrum processing

ANSIG v3.3 - Assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB EGA60263 EGA76627 EGA84535

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks