BMRB Entry 6145

Title:
The pyrophosphate cage: the structure of the nisin/lipid II complex provides a blueprint for novel antibiotics
Deposition date:
2004-03-16
Original release date:
2004-10-15
Authors:
Hsu, Shang-Te; Breukink, Eefjan; Tischenko, Eugene; Lutters, Mandy; de Kruijff, Ben; Kaptein, Robert; Bonvin, ALexandre; van Nuland, Nico
Citation:

Citation: Hsu, Shang-Te; Breukink, Eefjan; Tischenko, Eugene; Lutters, Mandy; de Kruijff, Ben; Kaptein, Robert; Bonvin, Alexandre; van Nuland, Nico. "The nisin-lipid II complex reveals a pyrophosphate cage that provides a blueprint for novel antibiotics."  Nat. Struct. Mol. Biol. 11, 963-967 (2004).
PubMed: 15361862

Assembly members:

Assembly members:
entity_3LII, polymer, 11 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1358   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: cell free synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_3LII: AXKXXXXXXXX

Data sets:
Data typeCount
1H chemical shifts62
31P chemical shifts2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
13LII1

Entities:

Entity 1, 3LII 11 residues - Formula weight is not available

1   ALADGLLYSDALDALNAGMUBPOPTPLiTPLi
2   TPLi

Samples:

sample_1: G-gamma-glutamic acid 1.2 mM; d6-DMSO 99%

Ex-cond_1: pH: . .; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availablenot available
1H-15N TOCSYsample_1not availablenot available
1H-1H NOESYsample_1not availablenot available
1H-1H TOCSYsample_1not availablenot available
1H-1H DQF-COSYsample_1not availablenot available

Software:

NMRPipe -

NMRView -

NMR spectrometers:

  • Bruker AVANCE 500 MHz
  • Bruker AVANCE 600 MHz
  • Bruker AVANVE 750 MHz
  • Bruker AVANVE 900 MHz