BMRB Entry 6143

Name: 1H and 15N assignments for human apolipoprotein C-II in DPC micelles
Published: 2005-03-14

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6143

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N assignments for human apolipoprotein C-II in DPC micelles

Deposition date:

2004-03-14

Original release date:

2005-03-14

Authors:

MacRaild, Christopher; Howlett, Geoffrey; Gooley, Paul

Citation:

Citation: MacRaild, Christopher; Howlett, Geoffrey; Gooley, Paul. "The Structure and Interactions of Human Apolipoprotein C-II in Dodecyl Phosphocholine" Biochemistry 43, 8084-8093 (2004).
PubMed: 15209504

Assembly members:

Assembly members:
apoC-II, polymer, 79 residues, 8916 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apoC-II: TQQPQQDEMPSPTFLTQVKE SLSSYWESAKTAAQNLYEKT YLPAVDEKLRDLYSKSTAAM STYTGIFTDQVLSVLKGEE

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	273
15N chemical shifts	66

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apolipoprotein C-II	1

Entities:

Entity 1, apolipoprotein C-II 79 residues - 8916 Da.

1

THR

GLN

PRO

GLN

ASP

GLU

MET

PRO

2

SER

PRO

THR

PHE

LEU

THR

GLN

VAL

LYS

GLU

3

SER

LEU

SER

TYR

TRP

GLU

SER

ALA

LYS

4

THR

ALA

GLN

ASN

LEU

TYR

GLU

LYS

THR

5

TYR

LEU

PRO

ALA

VAL

ASP

GLU

LYS

LEU

ARG

6

ASP

LEU

TYR

SER

LYS

SER

THR

ALA

MET

7

SER

THR

TYR

THR

GLY

ILE

PHE

THR

ASP

GLN

8

VAL

LEU

SER

VAL

LEU

LYS

GLY

GLU

Samples:

sample_1: apoC-II, [U-15N], 1.8 mM; DPC 85 mM; sodium acetate 20 mM; sodium azide 0.1 mM

Ex-cond_1: pH: 5.0; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
HMQC	sample_1	not available	Ex-cond_1
1H-15N NOESY	sample_1	not available	Ex-cond_1
1H-15C TOCSY	sample_1	not available	Ex-cond_1
HNHA	sample_1	not available	Ex-cond_1
HNHB	sample_1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Varian Inova 500 MHz

BMRB	5534
PDB	1I5J 1O8T 1SOH
EMBL	CAA25234 CAA28798
GB	AAA51743 AAA98743 AAB21042 AAB59380 AAH05348
PRF	1303321A
REF	NP_000474 NP_001230828 XP_008969010
SP	P02655
AlphaFold	P02655