BMRB Entry 6139

Name: Solution Structure of Ole e 6, an allergen from olive tree pollen
Published: 2004-08-05

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PDB ID: 1ss3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6139
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of Ole e 6, an allergen from olive tree pollen

Deposition date:

2004-03-11

Original release date:

2004-08-05

Authors:

Trevino, Miguel; Garcia-Mayoral, M Flor; Barral, Patricia; Villalba, Mayte; Rico, Manuel; Santoro, Jorge; Rodriguez, Rosalia; Bruix, Marta

Citation:

Citation: Trevino, Miguel; Garcia-Mayoral, M. Flor; Barral, Patricia; Villalba, Mayte; Santoro, Jorge; Rico, Manuel; Rodriguez, Rosalia; Bruix, Marta. "NMR Solution Structure of Ole e 6, a major allergen from olive tree pollen" J. Biol. Chem. ., .-. (2004).
PubMed: 15247256

Assembly members:

Assembly members:
Olea europea pollen allergen ole e 6, polymer, 50 residues, 5810 Da.

Natural source:

Natural source: Common Name: olive tree Taxonomy ID: 4146 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Olea europea

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPIC9

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Olea europea pollen allergen ole e 6: DEAQFKECYDTCHKECSDKG NGFTFCEMKCDTDCSVKDVK EKLENYKPKN

Data sets:

assigned_chemical_shifts
- cs_olee6_H2O
- cs_olee6_D2O

Data type	Count
15N chemical shifts	52
1H chemical shifts	614

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ole e 6	1

Entities:

Entity 1, ole e 6 50 residues - 5810 Da.

1	ASP	GLU	ALA	GLN	PHE	LYS	GLU	CYS	TYR	ASP
2	THR	CYS	HIS	LYS	GLU	CYS	SER	ASP	LYS	GLY
3	ASN	GLY	PHE	THR	PHE	CYS	GLU	MET	LYS	CYS
4	ASP	THR	ASP	CYS	SER	VAL	LYS	ASP	VAL	LYS
5	GLU	LYS	LEU	GLU	ASN	TYR	LYS	PRO	LYS	ASN

Samples:

sample_1: Olea europea pollen allergen ole e 60.6 – 1.0 mM; H2O 95%; D2O 5%

sample_2: Olea europea pollen allergen ole e 60.6 – 1.0 mM; D2O 100%

sample_3: Olea europea pollen allergen ole e 6, [U-15N], 0.6 – 1.0 mM; H2O 95%; D2O 5%

cond_pH6_H2O: pH: 6.0; temperature: 308 K

cond_pH6_D2O: pH: 6.0; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-COSY	not available	not available	not available
1H-TOCSY	not available	not available	not available
1H-NOESY	not available	not available	not available
1H-15N-HSQC	not available	not available	not available
1H-15N-NOESY-HSQC	not available	not available	not available

Software:

XWINNMR - raw spectral data proccesing

ANSIG v3.3 - crosspeak area calculations, peak assigments, peak picking

CALIBA - upper limits calculations

DYANA v1.5 - structures calculations

GLOMSA - stereospecific assigments

NMR spectrometers:

Bruker Avance 600 MHz

PDB	1SS3
GenBank	AAB66909 AAO33896 AAO34670
SWISS-PROT	O24172

1	ASP	GLU	ALA	GLN	PHE	LYS	GLU	CYS	TYR	ASP
2	THR	CYS	HIS	LYS	GLU	CYS	SER	ASP	LYS	GLY
3	ASN	GLY	PHE	THR	PHE	CYS	GLU	MET	LYS	CYS
4	ASP	THR	ASP	CYS	SER	VAL	LYS	ASP	VAL	LYS
5	GLU	LYS	LEU	GLU	ASN	TYR	LYS	PRO	LYS	ASN

1	ASP	GLU	ALA	GLN	PHE	LYS	GLU	CYS	TYR	ASP
2	THR	CYS	HIS	LYS	GLU	CYS	SER	ASP	LYS	GLY
3	ASN	GLY	PHE	THR	PHE	CYS	GLU	MET	LYS	CYS
4	ASP	THR	ASP	CYS	SER	VAL	LYS	ASP	VAL	LYS
5	GLU	LYS	LEU	GLU	ASN	TYR	LYS	PRO	LYS	ASN