BMRB Entry 6136

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6136

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Title:
Backbone Resonance Assignment of the L-Arabinose Binding Protein in Complex with D-Galactose
Deposition date:
2004-03-08
Original release date:
2004-06-25
Authors:
Hernandez Daranas, Antonio; Kalverda, Arnout; Chiovitty, Anthony; Homans, Steve
Citation:

Citation: Hernandez Daranas, Antonio; Kalverda, Arnout; Chiovitty, Anthony; Homans, Steve. "Backbone Resonance Assignment of the L-Arabinose Binding Protein in Complex with D-Galactose"  J. Biomol. NMR 28, 191-192 (2004).
PubMed: 14755164

Assembly members:

Assembly members:
L-Arabinose Binding Protein, polymer, 306 residues, Formula weight is not available
GAL, non-polymer, 180.156 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
L-Arabinose Binding Protein: ENLKLGFLVKQPEEPWFQTE WKFADKAGKDLGFEVIKIAV PDGEKTLNAIDSLAASGAKG FVICTPDPKLGSAIVAKARG YDMKVIAVDDQFVNAKGKPM DTVPLVMMAATKIGERQGQE LYKEMQKRGWDVKESAVMAI TANELDTARRRTTGSMDALK AAGFPEKQIYQVPTKSNDIP GAFDAANSMLVQHPEVKHWL IVGMNDSTVLGGVRATEGQG FKAADIIGIGINGVDAVSEL SKAQATGFYGSLLPSPDVHG YKSSEMLYNWVAKDVEPPKF TEVTDVVLITRDNFKEELEK KGLGGK

Data sets:
Data typeCount
13C chemical shifts818
1H chemical shifts270
15N chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ABP1
2D-galactose2

Entities:

Entity 1, ABP 306 residues - Formula weight is not available

1   GLUASNLEULYSLEUGLYPHELEUVALLYS
2   GLNPROGLUGLUPROTRPPHEGLNTHRGLU
3   TRPLYSPHEALAASPLYSALAGLYLYSASP
4   LEUGLYPHEGLUVALILELYSILEALAVAL
5   PROASPGLYGLULYSTHRLEUASNALAILE
6   ASPSERLEUALAALASERGLYALALYSGLY
7   PHEVALILECYSTHRPROASPPROLYSLEU
8   GLYSERALAILEVALALALYSALAARGGLY
9   TYRASPMETLYSVALILEALAVALASPASP
10   GLNPHEVALASNALALYSGLYLYSPROMET
11   ASPTHRVALPROLEUVALMETMETALAALA
12   THRLYSILEGLYGLUARGGLNGLYGLNGLU
13   LEUTYRLYSGLUMETGLNLYSARGGLYTRP
14   ASPVALLYSGLUSERALAVALMETALAILE
15   THRALAASNGLULEUASPTHRALAARGARG
16   ARGTHRTHRGLYSERMETASPALALEULYS
17   ALAALAGLYPHEPROGLULYSGLNILETYR
18   GLNVALPROTHRLYSSERASNASPILEPRO
19   GLYALAPHEASPALAALAASNSERMETLEU
20   VALGLNHISPROGLUVALLYSHISTRPLEU
21   ILEVALGLYMETASNASPSERTHRVALLEU
22   GLYGLYVALARGALATHRGLUGLYGLNGLY
23   PHELYSALAALAASPILEILEGLYILEGLY
24   ILEASNGLYVALASPALAVALSERGLULEU
25   SERLYSALAGLNALATHRGLYPHETYRGLY
26   SERLEULEUPROSERPROASPVALHISGLY
27   TYRLYSSERSERGLUMETLEUTYRASNTRP
28   VALALALYSASPVALGLUPROPROLYSPHE
29   THRGLUVALTHRASPVALVALLEUILETHR
30   ARGASPASNPHELYSGLUGLULEUGLULYS
31   LYSGLYLEUGLYGLYLYS

Entity 2, D-galactose - C6 H12 O6 - 180.156 Da.

1   GAL

Samples:

sample_1: L-Arabinose Binding Protein, [U-15N; U-13C; U-50% 2H], 1.5 mM; D-GALACTOSE 1.5 mM

Exp-cond_1: pH: 7.0; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableExp-cond_1
1H-15N NOESYsample_1not availableExp-cond_1
HNCAsample_1not availableExp-cond_1
HN(CO)CAsample_1not availableExp-cond_1
HNCOsample_1not availableExp-cond_1
HN(CA)COsample_1not availableExp-cond_1
HN(CA)CBsample_1not availableExp-cond_1
HN(COCA)CBsample_1not availableExp-cond_1

Software:

NMRPipe - data processing

NMRView v5.0.4 - peak picking, assignments

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA15721 BAB36032 BAG77657 BAI25989 BAI30952
EMBL CAA29476 CAP76386 CAQ32375 CAQ98838 CAR03257
GB AAC74971 AAG56890 AAN80773 AAZ87934 ABB61277
REF NP_288336 NP_310636 NP_416414 NP_754206 WP_000011369
SP P02924
AlphaFold P02924

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks