BMRB Entry 6133

Name: 1H, 13C, and 15N Chemical Shift Assignments for YojN-HPt
Published: 2004-09-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6133

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for YojN-HPt

Deposition date:

2004-03-01

Original release date:

2004-09-30

Authors:

Rogov, Vladimir; Loehr, Frank; Bernhard, Frank; Doetsch, Volker

Citation:

Citation: Rogov, Vladimir; Loehr, Frank; Bernhard, Frank; Doetsch, Volker. "Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the Escherichia coli YojN Histidine-Phosphotransferase (HPt) domain." J. Biomol. NMR 30, 103-104 (2004).
PubMed: 15452440

Assembly members:

Assembly members:
YojN-HPt, polymer, 116 residues, 12840 Da.

Natural source:

Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia Vector: M15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YojN-HPt: MQEAVLQLIEVQLAQEEVTE SPLGGDENAQLHASGYYALF VDTVPDDVKRLYTEAATSDF AALAQTAHRLKGVFAMLNLV PGKQLCETLEHLIREKDVPG IEKYISDIDSYVKSLL

Data sets:

assigned_chemical_shifts
- shift_1

Data type	Count
1H chemical shifts	784
13C chemical shifts	524
15N chemical shifts	121

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	YojN-HPt	1

Entities:

Entity 1, YojN-HPt 116 residues - 12840 Da.

1

MET

GLN

GLU

ALA

VAL

LEU

GLN

LEU

ILE

GLU

2

VAL

GLN

LEU

ALA

GLN

GLU

VAL

THR

GLU

3

SER

PRO

LEU

GLY

ASP

GLU

ASN

ALA

GLN

4

LEU

HIS

ALA

SER

GLY

TYR

ALA

LEU

PHE

5

VAL

ASP

THR

VAL

PRO

ASP

VAL

LYS

ARG

6

LEU

TYR

THR

GLU

ALA

THR

SER

ASP

PHE

7

ALA

LEU

ALA

GLN

THR

ALA

HIS

ARG

LEU

8

LYS

GLY

VAL

PHE

ALA

MET

LEU

ASN

LEU

VAL

9

PRO

GLY

LYS

GLN

LEU

CYS

GLU

THR

LEU

GLU

10

HIS

LEU

ILE

ARG

GLU

LYS

ASP

VAL

PRO

GLY

11

ILE

GLU

LYS

TYR

ILE

SER

ASP

ILE

ASP

SER

12

TYR

VAL

LYS

SER

LEU

Samples:

sample_1: YojN-HPt 1 mM; Bis-Tris 15 mM; NaCl 15 mM; H2O 99.99%

sample_2: YojN-HPt, [U-95% 13C; U-95% 15N], 1 mM; Bis-Tris 15 mM; NaCl 15 mM; H2O 5%; D2O 95%

Conditions_1: pH: 7.0; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D [15N, 1H]-TROSY-HNCO	not available	not available	Conditions_1
3D (HCA)CO(CA)NH	not available	not available	Conditions_1
3D [15N, 1H]-TROSY-HNCACB	not available	not available	Conditions_1
3D HBHA(CBCA)(CO)NH	not available	not available	Conditions_1
3D H(CC)(CO)NH-TOCSY	not available	not available	Conditions_1
3D (H)C(C)(CO)NH-TOCSY	not available	not available	Conditions_1
3D 15N-edited TOCSY-HSQC	not available	not available	Conditions_1
2D TOCSY in 2H2O	not available	not available	Conditions_1

Software:

No software information available

NMR spectrometers:

Bruker DMX 500 MHz
Bruker DMX 600 MHz
Bruker Avance 700 MHz

PDB	1SR2
DBJ	BAA15999 BAB36528 BAG78008 BAI26355 BAI31463
EMBL	CAP76719 CAQ99144 CAR03645 CAR08863 CAR13737
GB	AAC75276 AAG57351 AAN43818 AAN81213 AAP17635
REF	NP_288796 NP_311132 NP_416720 NP_708111 NP_754645
SP	P39838
AlphaFold	P39838