BMRB Entry 6133

Title:
1H, 13C, and 15N Chemical Shift Assignments for YojN-HPt
Deposition date:
2004-03-01
Original release date:
2004-09-30
Authors:
Rogov, Vladimir; Loehr, Frank; Bernhard, Frank; Doetsch, Volker
Citation:

Citation: Rogov, Vladimir; Loehr, Frank; Bernhard, Frank; Doetsch, Volker. "Letter to the Editor: Assignment of 1H, 13C and 15N resonances of the Escherichia coli YojN Histidine-Phosphotransferase (HPt) domain."  J. Biomol. NMR 30, 103-104 (2004).
PubMed: 15452440

Assembly members:

Assembly members:
YojN-HPt, polymer, 116 residues, 12840 Da.

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia   Vector: M15

Data sets:
Data typeCount
1H chemical shifts784
13C chemical shifts524
15N chemical shifts121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YojN-HPt1

Entities:

Entity 1, YojN-HPt 116 residues - 12840 Da.

1   METGLNGLUALAVALLEUGLNLEUILEGLU
2   VALGLNLEUALAGLNGLUGLUVALTHRGLU
3   SERPROLEUGLYGLYASPGLUASNALAGLN
4   LEUHISALASERGLYTYRTYRALALEUPHE
5   VALASPTHRVALPROASPASPVALLYSARG
6   LEUTYRTHRGLUALAALATHRSERASPPHE
7   ALAALALEUALAGLNTHRALAHISARGLEU
8   LYSGLYVALPHEALAMETLEUASNLEUVAL
9   PROGLYLYSGLNLEUCYSGLUTHRLEUGLU
10   HISLEUILEARGGLULYSASPVALPROGLY
11   ILEGLULYSTYRILESERASPILEASPSER
12   TYRVALLYSSERLEULEU

Samples:

sample_1: YojN-HPt 1 mM; Bis-Tris 15 mM; NaCl 15 mM; H2O 99.99%

sample_2: YojN-HPt, [U-95% 13C; U-95% 15N], 1 mM; Bis-Tris 15 mM; NaCl 15 mM; H2O 5%; D2O 95%

Conditions_1: pH: 7.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D [15N, 1H]-TROSY-HNCOnot availablenot availableConditions_1
3D (HCA)CO(CA)NHnot availablenot availableConditions_1
3D [15N, 1H]-TROSY-HNCACBnot availablenot availableConditions_1
3D HBHA(CBCA)(CO)NHnot availablenot availableConditions_1
3D H(CC)(CO)NH-TOCSYnot availablenot availableConditions_1
3D (H)C(C)(CO)NH-TOCSYnot availablenot availableConditions_1
3D 15N-edited TOCSY-HSQCnot availablenot availableConditions_1
2D TOCSY in 2H2Onot availablenot availableConditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAA15999 BAB36528 BAG78008 BAI26355 BAI31463
EMBL CAP76719 CAQ99144 CAR03645 CAR08863 CAR13737
GB AAC75276 AAG57351 AAN43818 AAN81213 AAP17635
REF NP_288796 NP_311132 NP_416720 NP_708111 NP_754645
SP P39838
AlphaFold P39838

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks