BMRB Entry 6132

Name: Letter to the Editor: 1H, 13C and 15N NMR assignment of the homodimeric poplar phloem peroxiredoxin
Published: 2004-09-30

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6132

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Letter to the Editor: 1H, 13C and 15N NMR assignment of the homodimeric poplar phloem peroxiredoxin

Deposition date:

2004-03-04

Original release date:

2004-09-30

Authors:

Bouillac, Sandrine; Rouhier, Nicolas; Tsan, Pascale; Jacquot, Jean-Pierre; Lancelin, Jean-Marc

Citation:

Citation: Bouillac, Sandrine; Rouhier, Nicolas; Tsan, Pascale; Jacquot, Jean-Pierre; Lancelin, Jean-Marc. "Letter to the Editor: 1H, 13C and 15N NMR assignment of the homodimeric poplar phloem type II peroxiredoxin" J. Biomol. NMR 30, 105-106 (2004).
PubMed: 15452441

Assembly members:

Assembly members:
peroxiredoxin, polymer, 162 residues, 17408 Da.

Natural source:

Natural source: Common Name: poplar Taxonomy ID: 3689 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Populus trichocarpa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
peroxiredoxin: MAPIAVGDVLPDGKLAYFDE QDQLQEVSVHSLVAGKKVIL FGVPGAFTPTCSLKHVPGFI EKAGELKSKGVTEILCISVN DPFVMKAWAKSYPENKHVKF LADGSATYTHALGLELDLQG KGLGTRSRRFALLVDDLKVK AANIEGGGEFTVSSAEDILK DL

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	604
15N chemical shifts	151
1H chemical shifts	895

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	prx subunit 1	1
2	prx subunit 2	1

Entities:

Entity 1, prx subunit 1 162 residues - 17408 Da.

1

MET

ALA

PRO

ILE

ALA

VAL

GLY

ASP

VAL

LEU

2

PRO

ASP

GLY

LYS

LEU

ALA

TYR

PHE

ASP

GLU

3

GLN

ASP

GLN

LEU

GLN

GLU

VAL

SER

VAL

HIS

4

SER

LEU

VAL

ALA

GLY

LYS

VAL

ILE

LEU

5

PHE

GLY

VAL

PRO

GLY

ALA

PHE

THR

PRO

THR

6

CYS

SER

LEU

LYS

HIS

VAL

PRO

GLY

PHE

ILE

7

GLU

LYS

ALA

GLY

GLU

LEU

LYS

SER

LYS

GLY

8

VAL

THR

GLU

ILE

LEU

CYS

ILE

SER

VAL

ASN

9

ASP

PRO

PHE

VAL

MET

LYS

ALA

TRP

ALA

LYS

10

SER

TYR

PRO

GLU

ASN

LYS

HIS

VAL

LYS

PHE

11

LEU

ALA

ASP

GLY

SER

ALA

THR

TYR

THR

HIS

12

ALA

LEU

GLY

LEU

GLU

LEU

ASP

LEU

GLN

GLY

13

LYS

GLY

LEU

GLY

THR

ARG

SER

ARG

PHE

14

ALA

LEU

VAL

ASP

LEU

LYS

VAL

LYS

15

ALA

ASN

ILE

GLU

GLY

GLU

PHE

16

THR

VAL

SER

ALA

GLU

ASP

ILE

LEU

LYS

17

ASP

LEU

Samples:

sample_1: peroxiredoxin, [U-100% 15N; U-100% 13C; 50% 2H], 1.5 mM; potassium phosphate 50 mM

sample_2: peroxiredoxin, [U-100% 15N], 1.1 mM; potassium phosphate 50 mM

Ex-cond: pH: 7.2; temperature: 311 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N-HSQC	not available	not available	Ex-cond
1H-15N NOESY-HSQC	not available	not available	Ex-cond
HNCO	not available	not available	Ex-cond
HN(CA)CO	not available	not available	Ex-cond
HNCA	not available	not available	Ex-cond
HN(CO)CA	not available	not available	Ex-cond
HN(CA)CB	not available	not available	Ex-cond
HN(COCA)CB	not available	not available	Ex-cond
(H)C(CCO)NH-TOCSY	not available	not available	Ex-cond
H(CCO)NH-TOCSY	not available	not available	Ex-cond
1H-1H TOCSY	not available	not available	Ex-cond
1H-1H NOESY	not available	not available	Ex-cond

Software:

NMRPipe - processing

PIPP - assignment

NMR spectrometers:

Varian Inova 800 MHz
Varian Inova 600 MHz
Bruker Avance 500 MHz

PDB	1TP9
GenBank	AAL90751 ABK94117