BMRB Entry 6122

Name: 1H/13C/15N assignment of the S1 domain of RNase E of E. coli
Published: 2004-09-01

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PDB ID: 1slj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6122
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H/13C/15N assignment of the S1 domain of RNase E of E. coli

Deposition date:

2004-02-27

Original release date:

2004-09-01

Authors:

Schubert, Mario; Edge, Robert; Lario, Paula; Cook, Michael; Strynadka, Natalie; Mackie, George; McIntosh, Lawrence

Citation:

Citation: Schubert, Mario; Edge, Robert; Lario, Paula; Cook, Michael; Strynadka, Natalie; Mackie, George; McIntosh, Lawrence. "Structural Characterization of the RNase E S1 domain and identification of its Oligonucleotide-binding and Dimerization Interfaces" J. Mol. Biol. 341, 37-54 (2004).

Assembly members:

Assembly members:
S1 Rnase E, polymer, 96 residues, 10687 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S1 Rnase E: GSHMLEQKKANIYKGKITRI EPSLEAAFVDYGAERHGFLP LKEIAREYFPANYSAHGRPN IKDVLREGQEVIVQIDKEER GNKGAALTTFISLAGS

Data sets:

assigned_chemical_shifts
- chemical_shift

Data type	Count
1H chemical shifts	636
13C chemical shifts	389
15N chemical shifts	100

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	S1 Rnase E	1

Entities:

Entity 1, S1 Rnase E 96 residues - 10687 Da.

1

GLY

SER

HIS

MET

LEU

GLU

GLN

LYS

ALA

2

ASN

ILE

TYR

LYS

GLY

LYS

ILE

THR

ARG

ILE

3

GLU

PRO

SER

LEU

GLU

ALA

PHE

VAL

ASP

4

TYR

GLY

ALA

GLU

ARG

HIS

GLY

PHE

LEU

PRO

5

LEU

LYS

GLU

ILE

ALA

ARG

GLU

TYR

PHE

PRO

6

ALA

ASN

TYR

SER

ALA

HIS

GLY

ARG

PRO

ASN

7

ILE

LYS

ASP

VAL

LEU

ARG

GLU

GLY

GLN

GLU

8

VAL

ILE

VAL

GLN

ILE

ASP

LYS

GLU

ARG

9

GLY

ASN

LYS

GLY

ALA

LEU

THR

PHE

10

ILE

SER

LEU

ALA

GLY

SER

Samples:

sample_1: S1 Rnase E, [U-13C; U-15N], 1.5 mM

Ex-cond_1: pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCACB	sample_1	not available	Ex-cond_1
CBCA(CO)NH	sample_1	not available	Ex-cond_1
HNCO	sample_1	not available	Ex-cond_1
H(CCO)NH-TOCSY	sample_1	not available	Ex-cond_1
C(CO)NH-TOCSY	sample_1	not available	Ex-cond_1
HACAN	sample_1	not available	Ex-cond_1
CBHD	sample_1	not available	Ex-cond_1
CBHE	sample_1	not available	Ex-cond_1
EZ-HMQC-NH2	sample_1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Varian Inova 600 MHz
Varian Unity 500 MHz

PDB	1SLJ 1SMX 1SN8 2BX2 2C0B 2C4R 2VMK 2VRT
DBJ	BAA35893 BAB34885 BAG76671 BAH62786 BAI24718
EMBL	CAA38206 CAA47818 CAD08310 CAP75576 CAQ31605
GB	AAA23443 AAB27352 AAC74168 AAG55830 AAL20115
PIR	AC0641
REF	NP_287218 NP_309489 NP_415602 NP_455679 NP_460156
SP	P21513
AlphaFold	P21513 P21513