BMRB Entry 6118
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6118
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Citation: Noguera, Valerie; Rouhier, Nicolas; Krimm, Isabelle; Jacquot, Jean-Pierre; Lancelin, Jean-Marc. "1H, 13C and 15N resonance assignments of poplar phloem glutaredoxin" J. Biomol. NMR 30, 219-220 (2004).
Assembly members:
glutaredoxin, polymer, 112 residues, 12383 Da.
Natural source: Common Name: Poplar Taxonomy ID: 3694 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Populus trichocarpa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3d
Entity Sequences (FASTA):
glutaredoxin: AGSPEATFVKKTISSHQIVI
FSKSYCPYCKKAKGVFKELN
QTPHVVELDQREDGHDIQDA
MSEIVGRRTVPQVFIDGKHI
GGSDDTVEAYESGELAKLLG
VASEQKDDFKLE
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 671 |
| 13C chemical shifts | 214 |
| 15N chemical shifts | 108 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Grx monomer | 1 |
Entities:
Entity 1, Grx monomer 112 residues - 12383 Da.
| 1 | ALA | GLY | SER | PRO | GLU | ALA | THR | PHE | VAL | LYS | ||||
| 2 | LYS | THR | ILE | SER | SER | HIS | GLN | ILE | VAL | ILE | ||||
| 3 | PHE | SER | LYS | SER | TYR | CYS | PRO | TYR | CYS | LYS | ||||
| 4 | LYS | ALA | LYS | GLY | VAL | PHE | LYS | GLU | LEU | ASN | ||||
| 5 | GLN | THR | PRO | HIS | VAL | VAL | GLU | LEU | ASP | GLN | ||||
| 6 | ARG | GLU | ASP | GLY | HIS | ASP | ILE | GLN | ASP | ALA | ||||
| 7 | MET | SER | GLU | ILE | VAL | GLY | ARG | ARG | THR | VAL | ||||
| 8 | PRO | GLN | VAL | PHE | ILE | ASP | GLY | LYS | HIS | ILE | ||||
| 9 | GLY | GLY | SER | ASP | ASP | THR | VAL | GLU | ALA | TYR | ||||
| 10 | GLU | SER | GLY | GLU | LEU | ALA | LYS | LEU | LEU | GLY | ||||
| 11 | VAL | ALA | SER | GLU | GLN | LYS | ASP | ASP | PHE | LYS | ||||
| 12 | LEU | GLU |
Samples:
sample_1: glutaredoxin, [U-15N], 0.9 mM
sample_2: glutaredoxin, [U-13C ; U-15N], 0.9 mM
sample_3: glutaredoxin 0.9 mM
cond_1: pH: 7.2; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | cond_1 |
| HN(CO)CA | not available | not available | cond_1 |
| HNCO | not available | not available | cond_1 |
| 1H-15N NOESY | not available | not available | cond_1 |
| HNHA | not available | not available | cond_1 |
| 15N HSQC | not available | not available | cond_1 |
| NOESY | not available | not available | cond_1 |
| TOCSY | not available | not available | cond_1 |
| COSY | not available | not available | cond_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 800 MHz
- Bruker DRX-Avance 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks