BMRB Entry 6118

Name: 1H, 13C and 15N assignments of poplar phloem glutaredoxin
Published: 2004-11-29

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6118

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N assignments of poplar phloem glutaredoxin

Deposition date:

2004-03-01

Original release date:

2004-11-29

Authors:

Noguera, Valerie; Rouhier, Nicolas; Krimm, Isabelle; Jacquot, Jean-Pierre; Lancelin, Jean-Marc

Citation:

Citation: Noguera, Valerie; Rouhier, Nicolas; Krimm, Isabelle; Jacquot, Jean-Pierre; Lancelin, Jean-Marc. "1H, 13C and 15N resonance assignments of poplar phloem glutaredoxin" J. Biomol. NMR 30, 219-220 (2004).

Assembly members:

Assembly members:
glutaredoxin, polymer, 112 residues, 12383 Da.

Natural source:

Natural source: Common Name: Poplar Taxonomy ID: 3694 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Populus trichocarpa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
glutaredoxin: AGSPEATFVKKTISSHQIVI FSKSYCPYCKKAKGVFKELN QTPHVVELDQREDGHDIQDA MSEIVGRRTVPQVFIDGKHI GGSDDTVEAYESGELAKLLG VASEQKDDFKLE

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	671
13C chemical shifts	214
15N chemical shifts	108

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Grx monomer	1

Entities:

Entity 1, Grx monomer 112 residues - 12383 Da.

1

ALA

GLY

SER

PRO

GLU

ALA

THR

PHE

VAL

LYS

2

LYS

THR

ILE

SER

HIS

GLN

ILE

VAL

ILE

3

PHE

SER

LYS

SER

TYR

CYS

PRO

TYR

CYS

LYS

4

LYS

ALA

LYS

GLY

VAL

PHE

LYS

GLU

LEU

ASN

5

GLN

THR

PRO

HIS

VAL

GLU

LEU

ASP

GLN

6

ARG

GLU

ASP

GLY

HIS

ASP

ILE

GLN

ASP

ALA

7

MET

SER

GLU

ILE

VAL

GLY

ARG

THR

VAL

8

PRO

GLN

VAL

PHE

ILE

ASP

GLY

LYS

HIS

ILE

9

GLY

SER

ASP

THR

VAL

GLU

ALA

TYR

10

GLU

SER

GLY

GLU

LEU

ALA

LYS

LEU

GLY

11

VAL

ALA

SER

GLU

GLN

LYS

ASP

PHE

LYS

12

LEU

GLU

Samples:

sample_1: glutaredoxin, [U-15N], 0.9 mM

sample_2: glutaredoxin, [U-13C ; U-15N], 0.9 mM

sample_3: glutaredoxin 0.9 mM

cond_1: pH: 7.2; temperature: 301 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	cond_1
HN(CO)CA	not available	not available	cond_1
HNCO	not available	not available	cond_1
1H-15N NOESY	not available	not available	cond_1
HNHA	not available	not available	cond_1
15N HSQC	not available	not available	cond_1
NOESY	not available	not available	cond_1
TOCSY	not available	not available	cond_1
COSY	not available	not available	cond_1

Software:

No software information available

NMR spectrometers:

Varian INOVA 800 MHz
Bruker DRX-Avance 500 MHz

GB	AAL90750 ABK93676 ABK96111 EEF03801
REF	XP_002325236