BMRB Entry 6115

Name: Structural and Functional Analysis of a picornaviral Internal cis-acting replication element
Published: 2004-09-28

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6115

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structural and Functional Analysis of a picornaviral Internal cis-acting replication element

Deposition date:

2004-02-25

Original release date:

2004-09-28

Authors:

Kaluarachchi, Kumaralal; Thiviyanathan, Varatharasa

Citation:

Citation: Thiviyanathan, Varatharasa; Yang, Y.; Kaluarachchi, Kumaralal; Rijnbrand, R.; Gorenstein, D.; Lemon, S.. "High resolution Structure of a Picornaviral Internal Cis-acting RNA Replication Element (cre)" Proc. Natl. Acad. Sci. U.S.A. 101, 12688-12693 (2004).
PubMed: 15314212

Assembly members:

Assembly members:
Cis-acting replication element, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: viruses Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cis-acting replication element: GGUCAUCGUUGAGAAAACGA AACAGACGGUGGCC

Data sets:

assigned_chemical_shifts
- shift1

Data type	Count
1H chemical shifts	268

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cre	1

Entities:

Entity 1, cre 34 residues - Formula weight is not available

1

G

U

C

A

U

C

G

U

2

G

A

G

A

C

G

A

3

A

C

A

G

A

C

G

U

4

G

C

Samples:

sample_1: Cis-acting replication element, [U-13C; U-15N], 0.8 – 2.0 mM

ex-cond_1: pH: 6.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	ex-cond_1

Software:

FELIX v2000 - peak assignmnets, volume measurements

NMR spectrometers:

Varian UnityPlus 600 MHz
Varian UnityPlus 750 MHz