BMRB Entry 6114

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6114
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonance assignment of the C-terminal BRCT domain from human BRCA1
Deposition date:
2004-02-25
Original release date:
2005-02-28
Authors:
Gaiser, Olaf; Oschkinat, Hartmut; Heinemann, Udo; Ball, Linda
Citation:

Citation: Gaiser, Olaf; Oschkinat, Hartmut; Heinemann, Udo; Ball, Linda. "1H, 13C and 15N resonance assignment of the C-terminal BRCT domain from human BRCA1"  J. Biomol. NMR 30, 221-222 (2004).
PubMed: 15557806

Assembly members:

Assembly members:
C-terminal BRCT domain from human BRCA1, polymer, 110 residues, 12500 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal BRCT domain from human BRCA1: GSQDRKIFRGLEICCYGPFT NMPTDQLEWMVQLCGASVVK ELSSFTLGTGVHPIVVVQPD AWTEDNGFHAIGQMCEAPVV TREWVLDSVALYQCQELDTY LIPQIPHSHY

Data sets:
Data typeCount
1H chemical shifts736
13C chemical shifts470
15N chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1BRCTc1

Entities:

Entity 1, BRCTc 110 residues - 12500 Da.

1   GLYSERGLNASPARGLYSILEPHEARGGLY
2   LEUGLUILECYSCYSTYRGLYPROPHETHR
3   ASNMETPROTHRASPGLNLEUGLUTRPMET
4   VALGLNLEUCYSGLYALASERVALVALLYS
5   GLULEUSERSERPHETHRLEUGLYTHRGLY
6   VALHISPROILEVALVALVALGLNPROASP
7   ALATRPTHRGLUASPASNGLYPHEHISALA
8   ILEGLYGLNMETCYSGLUALAPROVALVAL
9   THRARGGLUTRPVALLEUASPSERVALALA
10   LEUTYRGLNCYSGLNGLULEUASPTHRTYR
11   LEUILEPROGLNILEPROHISSERHISTYR

Samples:

BRCTc_15N: C-terminal BRCT domain from human BRCA1, [U-15N], 1.3 mM

BRCTc_13C_15N: C-terminal BRCT domain from human BRCA1, [U-13C; U-15N], 0.7 mM

condition_Set_1: pH: 6.8; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
Spectrometers were in standard configuration, with triple resonance probesnot availablenot availablecondition_Set_1
equipped with self-shielded gradient coils.not availablenot availablecondition_Set_1

Software:

XWIN-NMR v1.3 - acquisition, processing of spectra

AZARA v2.1 - processing, viewing of spectra

ANSIG v3.3 - assignment, creation of NOE restraints lists

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAG10985 BAG57181
GB AAA73985 AAB08105 AAB61673 AAC00049 AAC37594
REF NP_001038958 NP_001108421 NP_001288295 NP_001288687 NP_001289032
SP P38398 Q6J6I8 Q6J6I9 Q6J6J0 Q9GKK8
AlphaFold P38398 Q6J6I8 Q6J6I9 Q6J6J0 Q9GKK8

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks