BMRB Entry 6112

Name: Backbone 1H, 13C and 15N Chemical Shift Assignments for p27-KID in Solution
Published: 2004-05-15

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6112

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C and 15N Chemical Shift Assignments for p27-KID in Solution

Deposition date:

2004-02-23

Original release date:

2004-05-15

Authors:

Lacy, Eilyn; Filippov, Igor; Lewis, William; Otieno, Steve; Xiao, Limin; Weiss, Sonja; Hengst, Ludger; Kriwacki, Richard

Citation:

Citation: Lacy, Eilyn; Filippov, Igor; Lewis, William; Otieno, Steve; Xiao, Limin; Weiss, Sonja; Hengst, Ludger; Kriwacki, Richard. "p27 binds cyclin-CDK complexes through a sequential mechanism involving binding-induced protein folding" Nat. Struct. Mol. Biol. 11, 358-364 (2004).
PubMed: 15024385

Assembly members:

Assembly members:
Cyclin dependent kinase inhibitor 1B, polymer, 88 residues, 10406 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia Coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cyclin dependent kinase inhibitor 1B: GSHMEHPKPSACRNLFGPVD HEELTRDLEKHCRDMEEASQ RKWNFDFQNHKPLEGKYEWQ EVEKGSLPEFYYRPPRPPKG ACKVPAQE

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	74
13C chemical shifts	82
15N chemical shifts	74

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p27-KID	1

Entities:

Entity 1, p27-KID 88 residues - 10406 Da.

1

GLY

SER

HIS

MET

GLU

HIS

PRO

LYS

PRO

SER

2

ALA

CYS

ARG

ASN

LEU

PHE

GLY

PRO

VAL

ASP

3

HIS

GLU

LEU

THR

ARG

ASP

LEU

GLU

LYS

4

HIS

CYS

ARG

ASP

MET

GLU

ALA

SER

GLN

5

ARG

LYS

TRP

ASN

PHE

ASP

PHE

GLN

ASN

HIS

6

LYS

PRO

LEU

GLU

GLY

LYS

TYR

GLU

TRP

GLN

7

GLU

VAL

GLU

LYS

GLY

SER

LEU

PRO

GLU

PHE

8

TYR

ARG

PRO

ARG

PRO

LYS

GLY

9

ALA

CYS

LYS

VAL

PRO

ALA

GLN

GLU

Samples:

p27KID: Cyclin dependent kinase inhibitor 1B, [U-2H; U-13C; U-15N], 1.0 mM

Cond_set_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
CT-HNCA	p27KID	not available	Cond_set_1
CT-HN(CO)CA	p27KID	not available	Cond_set_1
CT-HN(CA)CB	p27KID	not available	Cond_set_1
CT-HN(COCA)CB	p27KID	not available	Cond_set_1

Software:

Felix -

NMR spectrometers:

Varian Inova 600 MHz

PDB	1JSU
DBJ	BAG35368 BAG60584 BAG70105 BAG70237 BAG73064
EMBL	CAA59284 CAE82383 CAG33680 CAL37442 CAL38417
GB	AAA20240 AAF69497 AAH01971 AAL78041 AAV38358
REF	NP_004055 XP_002822998 XP_003808898 XP_004052799 XP_005680873
SP	P46527
AlphaFold	P46527