BMRB Entry 6108

Name: Solution structure of GlgS protein from E. coli
Published: 2004-06-30

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PDB ID: 1rrz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6108
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of GlgS protein from E. coli

Deposition date:

2004-02-20

Original release date:

2004-06-30

Authors:

Kozlov, G.; Gehring, K.

Citation:

Citation: Kozlov, G.; Elias, D.; Cygler, M.; Gehring, K.. "Structure of GlgS from Escherichia coli suggests a role in protein-protein interactions" BMC Biol. 2, 10-10 (2004).
PubMed: 15161493

Assembly members:

Assembly members:
glgS, polymer, 86 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
glgS: MGSSHHHHHHSSGLVPRGSH MDHSLNSLNNFDFLARSFAR MHAEGRPVDILAVTGNMDEE HRTWFCARYAWYCQQMMQAR ELELEH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	424
13C chemical shifts	188
15N chemical shifts	63

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	glgS	1

Entities:

Entity 1, glgS 86 residues - Formula weight is not available

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

ASP

HIS

SER

LEU

ASN

SER

LEU

ASN

4

PHE

ASP

PHE

LEU

ALA

ARG

SER

PHE

ALA

ARG

5

MET

HIS

ALA

GLU

GLY

ARG

PRO

VAL

ASP

ILE

6

LEU

ALA

VAL

THR

GLY

ASN

MET

ASP

GLU

7

HIS

ARG

THR

TRP

PHE

CYS

ALA

ARG

TYR

ALA

8

TRP

TYR

CYS

GLN

MET

GLN

ALA

ARG

9

GLU

LEU

GLU

LEU

GLU

HIS

Samples:

sample_1: glgS, [U-13C; U-15N], 1 mM; potassium phosphate 5 mM; DTT 1 mM; sodium azide 0.1 mM; H2O 90%; D2O 10%

sample_2: glgS 1 mM; potassium phosphate 5 mM; DTT 1 mM; sodium azide 0.1 mM; H2O 90%; D2O 10%

sample_3: glgS 1 mM; potassium phosphate 5 mM; DTT 1 mM; sodium azide 0.1 mM; D2O 100%

sample_cond_1: pH: 6.7; temperature: 298 K; ionic strength: 5 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	sample_cond_1
2D NOESY	not available	not available	sample_cond_1

Software:

XWINNMR v2.1 - collection, processing

XEASY v1.3.13 - data analysis

CYANA v1.0.6 - structure solution

Xplor-NIH v2.9.2 - refinement

NMR spectrometers:

Bruker AVANCE 500 MHz

PDB	1RRZ
DBJ	BAA16577 BAB37354 BAG78852 BAI27331 BAI32446
EMBL	CAA77940 CAP77523 CAQ33386 CAR00010 CAR04675
GB	AAC76085 AAG58183 AAN44566 AAN82242 AAP18379
REF	NP_289624 NP_311958 NP_417521 NP_708859 NP_755668
SP	A7ZRT0 A8A4K4 B1IRR7 B1LF40 B1XG54
AlphaFold	A7ZRT0 A8A4K4 B1IRR7 B1LF40 B1XG54