BMRB Entry 6103

Title:
Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha   PubMed: 15155727
Deposition date:
2004-02-19
Original release date:
2005-03-01
Authors:
Weljie, A.; Vogel, H.
Citation:

Citation: Weljie, A.; Vogel, H.. "Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha"  J. Biol. Chem. 279, 35494-35502 (2004).

Assembly members:

Assembly members:
Calcium-dependent protein kinase SK5, polymer, 87 residues, 40.078 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: soybean   Taxonomy ID: 3847   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Glycine max

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-19B

Experimental source:

Natural source:   Common Name: soybean   Taxonomy ID: 3847   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Glycine max

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-19B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calcium-dependent protein kinase SK5: HSSGHIDDDDKHMAERLSEE EIGGLKELFKMIDTDNSGTI TFDELKDGLKRVGSELMESE IKDLMDAADIDKSGTIDYGE FIAATVH

Data sets:
Data typeCount
1H chemical shifts437
15N chemical shifts80
13C chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Calcium-dependent protein kinase SK51
2CALCIUM (II) ION 12
3CALCIUM (II) ION 22

Entities:

Entity 1, Calcium-dependent protein kinase SK5 87 residues - 40.078 Da.

1   HISSERSERGLYHISILEASPASPASPASP
2   LYSHISMETALAGLUARGLEUSERGLUGLU
3   GLUILEGLYGLYLEULYSGLULEUPHELYS
4   METILEASPTHRASPASNSERGLYTHRILE
5   THRPHEASPGLULEULYSASPGLYLEULYS
6   ARGVALGLYSERGLULEUMETGLUSERGLU
7   ILELYSASPLEUMETASPALAALAASPILE
8   ASPLYSSERGLYTHRILEASPTYRGLYGLU
9   PHEILEALAALATHRVALHIS

Entity 2, CALCIUM (II) ION 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: Calcium-dependent protein kinase SK5, [U-15N], 0.5 mM; NaCl 200 mM; CaCl2 10 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 6.9; temperature: 303 K; ionic strength: 210 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablesample_cond_1

Software:

XWINNMR v2.6 - collection

NMRPipe v2.1 - processing

NMRView v5.04 - data analysis

CNS v1.1 - structure solution, refinement

ARIA v1.2 - iterative matrix relaxation

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
GB AAK38161

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks