BMRB Entry 6103
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6103
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Weljie, A.; Vogel, H.. "Unexpected structure of the Ca2+-regulatory region from soybean calcium-dependent protein kinase-alpha" J. Biol. Chem. 279, 35494-35502 (2004).
PubMed: 15155727
Assembly members:
Calcium-dependent protein kinase SK5, polymer, 87 residues, 40.078 Da.
CA, non-polymer, 40.078 Da.
Natural source: Common Name: soybean Taxonomy ID: 3847 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Glycine max
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET-19B
Entity Sequences (FASTA):
Calcium-dependent protein kinase SK5: HSSGHIDDDDKHMAERLSEE
EIGGLKELFKMIDTDNSGTI
TFDELKDGLKRVGSELMESE
IKDLMDAADIDKSGTIDYGE
FIAATVH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 437 |
| 15N chemical shifts | 80 |
| 13C chemical shifts | 202 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Calcium-dependent protein kinase SK5 | 1 |
| 2 | CALCIUM (II) ION 1 | 2 |
| 3 | CALCIUM (II) ION 2 | 2 |
Entities:
Entity 1, Calcium-dependent protein kinase SK5 87 residues - 40.078 Da.
| 1 | HIS | SER | SER | GLY | HIS | ILE | ASP | ASP | ASP | ASP | ||||
| 2 | LYS | HIS | MET | ALA | GLU | ARG | LEU | SER | GLU | GLU | ||||
| 3 | GLU | ILE | GLY | GLY | LEU | LYS | GLU | LEU | PHE | LYS | ||||
| 4 | MET | ILE | ASP | THR | ASP | ASN | SER | GLY | THR | ILE | ||||
| 5 | THR | PHE | ASP | GLU | LEU | LYS | ASP | GLY | LEU | LYS | ||||
| 6 | ARG | VAL | GLY | SER | GLU | LEU | MET | GLU | SER | GLU | ||||
| 7 | ILE | LYS | ASP | LEU | MET | ASP | ALA | ALA | ASP | ILE | ||||
| 8 | ASP | LYS | SER | GLY | THR | ILE | ASP | TYR | GLY | GLU | ||||
| 9 | PHE | ILE | ALA | ALA | THR | VAL | HIS |
Entity 2, CALCIUM (II) ION 1 - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: Calcium-dependent protein kinase SK5, [U-15N], 0.5 mM; NaCl 200 mM; CaCl2 10 mM; H2O 95%; D2O 5%
sample_cond_1: pH: 6.9; temperature: 303 K; ionic strength: 210 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | sample_cond_1 |
Software:
XWINNMR v2.6 - collection
NMRPipe v2.1 - processing
NMRView v5.04 - data analysis
CNS v1.1 - structure solution, refinement
ARIA v1.2 - iterative matrix relaxation
NMR spectrometers:
- Bruker AVANCE 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks