BMRB Entry 6098

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6098

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N chemical shift assignments for the amino terminal domain of the transcriptional cofactor PC4

Deposition date:

2004-02-13

Original release date:

2004-06-25

Authors:

Jonker, Henry; Folkers, Gert; Wechselberger, Rainer; Boelens, Rolf; Kaptein, Rob

Citation:

Citation: Jonker, Henry. "Structural and functional studies on transcriptional activation - Interactions of the cofactor PC4 and activator VP16" Thesis Utrecht University (2003).

Assembly members:

Assembly members:
positive cofactor 4, polymer, 126 residues, 14264 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
positive cofactor 4: PKSKELVSSSSSGSDSDSEV DKKLKRKKQVAPEKPVKKQK TGETSRALSSSKQSSSSRDD NMFQIGKMRYVSVRDFKGKV LIDIREYWMDPEGEMKPGRK GISLNPEQWSQLKEQISDID DAVRKL

Data sets:

assigned_chemical_shifts

Data type	Count
13C chemical shifts	492
1H chemical shifts	217
15N chemical shifts	159

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PC4ntd, 1	1
2	PC4ctd, 2	1

Entities:

Entity 1, PC4ntd, 1 126 residues - 14264 Da.

1

PRO

LYS

SER

LYS

GLU

LEU

VAL

SER

2

SER

GLY

SER

ASP

SER

ASP

SER

GLU

VAL

3

ASP

LYS

LEU

LYS

ARG

LYS

GLN

VAL

4

ALA

PRO

GLU

LYS

PRO

VAL

LYS

GLN

LYS

5

THR

GLY

GLU

THR

SER

ARG

ALA

LEU

SER

6

SER

LYS

GLN

SER

ARG

ASP

7

ASN

MET

PHE

GLN

ILE

GLY

LYS

MET

ARG

TYR

8

VAL

SER

VAL

ARG

ASP

PHE

LYS

GLY

LYS

VAL

9

LEU

ILE

ASP

ILE

ARG

GLU

TYR

TRP

MET

ASP

10

PRO

GLU

GLY

GLU

MET

LYS

PRO

GLY

ARG

LYS

11

GLY

ILE

SER

LEU

ASN

PRO

GLU

GLN

TRP

SER

12

GLN

LEU

LYS

GLU

GLN

ILE

SER

ASP

ILE

ASP

13

ASP

ALA

VAL

ARG

LYS

LEU

Samples:

sample_PC4_298K_LS: positive cofactor 4, [U-13C; U-15N], 0.5 – 1 mM; KCl 50 mM; KHxPO4 50 mM; D5-Glycin 2 M; Protease Inhibitor cocktail mM

sample_PC4_305K_HS: positive cofactor 4, [U-13C; U-15N], 0.5 – 1 mM; KCl 400 mM; KHxPO4 50 mM; D5-Glycin 2 M; Protease Inhibitor cocktail mM

sample_PC4_P: positive cofactor 4, [U-13C; U-15N], 0.5 – 1 mM; KCl 400 mM; KHxPO4 50 mM; D5-Glycin 2 M; Protease Inhibitor cocktail mM

condition_PC4_298K_LS: pH: 5.6; temperature: 298 K

condition_PC4_305K_HS: pH: 5.6; temperature: 305 K

condition_PC4_P: pH: 5.6; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D (1H,15N)-HSQC	not available	not available	not available
3D NOESY (1H,15N) HSQC	not available	not available	not available
3D TOCSY (1H,15N) HSQC	not available	not available	not available
3D HNCACB	not available	not available	not available
3D HNCO	not available	not available	not available
3D HN(CA)CO	not available	not available	not available

Software:

NMRPipe - processing

SPARKY v3 - analyses, assignment

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz
Bruker Avance 750 MHz

PDB	1PCF 2C62 2PHE
DBJ	BAD92522 BAE00374 BAE87869 BAE91489 BAG10763
EMBL	CAA56200 CAG33183 CAH92686
GB	AAA20980 AAH09610 AAH10537 AAH18189 AAH22339
REF	NP_001098877 NP_001126574 NP_001248140 NP_001272042 NP_006704
SP	P53999 Q4R947 Q5R6D0
TPG	DAA17835
AlphaFold	P53999 Q4R947 Q5R6D0