BMRB Entry 6097

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6097

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Proton chemical shift assignments for human spliceosomal protein U1C zinc finger domain

Deposition date:

2004-02-10

Original release date:

2004-09-28

Authors:

Muto, Yutaka; Pomeranz Krummel, Daniel; Oubridge, Chris; Hernandez, Helena; Robinson, Carol; Neuhaus, David; Nagai, Kiyoshi

Citation:

Citation: Muto, Yutaka; Pomeranz Krummel, Daniel; Oubridge, Chris; Hernandez, Helena; Robinson, Carol; Neuhaus, David; Nagai, Kiyoshi. "The structure and biochemical properties of the human spliceosomal protein U1C" J. Mol. Biol. 341, 185-198 (2004).
PubMed: 15312772

Assembly members:

Assembly members:
U1C protein, polymer, 61 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U1C protein: MPKFYCDYCDTYLTHDSPSV RKTHCSGRKHKENVKDYYQK WMEEQAQSLIDKTTAAFQQG K

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	377

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	U1C zinc finger domain	1
2	ZINC (II) ION	2

Entities:

Entity 1, U1C zinc finger domain 61 residues - Formula weight is not available

1

MET

PRO

LYS

PHE

TYR

CYS

ASP

TYR

CYS

ASP

2

THR

TYR

LEU

THR

HIS

ASP

SER

PRO

SER

VAL

3

ARG

LYS

THR

HIS

CYS

SER

GLY

ARG

LYS

HIS

4

LYS

GLU

ASN

VAL

LYS

ASP

TYR

GLN

LYS

5

TRP

MET

GLU

GLN

ALA

GLN

SER

LEU

ILE

6

ASP

LYS

THR

ALA

PHE

GLN

GLY

7

LYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: U1C protein0.8 – 1.0 mM; potassium phosphate 20 mM; magnesium sulphate 100 mM; sodium bromide 400 mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
TOCSY	sample_1	not available	Ex-cond_1
DQF-COSY	sample_1	not available	Ex-cond_1
NOESY	sample_1	not available	Ex-cond_1

Software:

xwin-nmr v3.0 - data processing

SPARKY - analysis

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2VRD 3CW1 4PJO
DBJ	BAE02052 BAE23273 BAE40776 BAG34895 BAI46833
EMBL	CAA31037 CAA45354 CAA65542 CAG11833 CBN80717
GB	AAA36618 AAB08894 AAH08243 AAH84206 AAH91442
PIR	I56013
REF	NP_001016598 NP_001069802 NP_001084130 NP_001158541 NP_001177132
SP	B0JYS7 D3ZCL3 E1C6F0 E2RGI3 F6TFD9
TPG	DAA16701
AlphaFold	B0JYS7 D3ZCL3 E1C6F0 E2RGI3 F6TFD9