BMRB Entry 6090

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6090
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonance assignement of the reduced form of methionine sulfoxide reductase A from Escherichia coli
Deposition date:
2004-02-04
Original release date:
2005-04-29
Authors:
Coudevylle, Nicolas; Thureau, Aurelien; Azza, Said; Boshi-Muller, Sandrine; Branlant, Guy; Cung, Manh-Thong
Citation:

Citation: Coudevylle, Nicolas; Thureau, Aurelien; Azza, Said; Boshi-Muller, Sandrine; Branlant, Guy; Cung, Manh-Thong. "Letter to the Editor: 1H, 13C and 15N resonance assignement of the reduced form of methionine sulfoxide reductase A from Escherichia coli"  J. Biomol. NMR 30, 363-364 (2004).

Assembly members:

Assembly members:
Methionine sulfoxide reductase A, polymer, 211 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Esherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Esherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Methionine sulfoxide reductase A: SLFDKKHLVSPADALPGRNT PMPVATLHAVNGHSMTNVPD GMEIAIFAMGCFWGVERLFW QLPGVYSTAAGYTGGYTPNP TYREVCSGDTGHAEAVRIVY DPSVISYEQLLQVFWENHDP AQGMRQGNDHGTQYRSAIYP LTPEQDAAARASLERFQAAM LAADDDRHITTEIANATPFY YAEDDHQQYLHKNPYGYCGI GGIGVCLPPEA

Data sets:
Data typeCount
1H chemical shifts795
13C chemical shifts651
15N chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MsrA1

Entities:

Entity 1, MsrA 211 residues - Formula weight is not available

1   SERLEUPHEASPLYSLYSHISLEUVALSER
2   PROALAASPALALEUPROGLYARGASNTHR
3   PROMETPROVALALATHRLEUHISALAVAL
4   ASNGLYHISSERMETTHRASNVALPROASP
5   GLYMETGLUILEALAILEPHEALAMETGLY
6   CYSPHETRPGLYVALGLUARGLEUPHETRP
7   GLNLEUPROGLYVALTYRSERTHRALAALA
8   GLYTYRTHRGLYGLYTYRTHRPROASNPRO
9   THRTYRARGGLUVALCYSSERGLYASPTHR
10   GLYHISALAGLUALAVALARGILEVALTYR
11   ASPPROSERVALILESERTYRGLUGLNLEU
12   LEUGLNVALPHETRPGLUASNHISASPPRO
13   ALAGLNGLYMETARGGLNGLYASNASPHIS
14   GLYTHRGLNTYRARGSERALAILETYRPRO
15   LEUTHRPROGLUGLNASPALAALAALAARG
16   ALASERLEUGLUARGPHEGLNALAALAMET
17   LEUALAALAASPASPASPARGHISILETHR
18   THRGLUILEALAASNALATHRPROPHETYR
19   TYRALAGLUASPASPHISGLNGLNTYRLEU
20   HISLYSASNPROTYRGLYTYRCYSGLYILE
21   GLYGLYILEGLYVALCYSLEUPROPROGLU
22   ALA

Samples:

sample_1: Methionine sulfoxide reductase A, [U-99% 13C; U-99% 15N], 0.9 mM

Ex-cond_1: pH: 7.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CBCANHnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

XWINNMR v3.1 - spectra processing

XEASY - peak peaking, spectra assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 6786
PDB
DBJ BAB38620 BAE78220 BAG80049 BAI28524 BAI33698
EMBL CAP78740 CAQ34570 CAR01194 CAR05961 CAR11177
GB AAA24399 AAA97115 AAC77176 AAG59417 AAN45686
REF NP_290851 NP_313224 NP_418640 NP_709979 NP_757164
SP A7ZV98 A8A7X5 B1ISY2 B1LRA3 B1XDW8
AlphaFold A7ZV98 A8A7X5 B1ISY2 B1LRA3 B1XDW8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks