BMRB Entry 6074

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6074

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Title:
Backbone 1H, 13C and 15N resonance assignments for the 29kD Pseudomonas aeruginosa dimethylarginine dimethylaminohydrolase involved in nitric oxide synthase regulation
Deposition date:
2004-01-13
Original release date:
2004-07-06
Authors:
S. Magalhaes, Beatriz; Harris, Richard; Plevin, Michael; Driscoll, Paul
Citation:

Citation: Magalhaes, Beatriz; Harris, Richard; Plevin, Michael; Driscoll, Paul. "Letter to the Editor: Backbone 1H, 13C and 15N resonance assignments for a 29kD Monomeric Variant of Pseudomonas aeruginosa dimethylarginine dimethylaminohydrolase "  J. Biomol. NMR 29, 463-464 (2004).
PubMed: 15213470

Assembly members:

Assembly members:
Pseudomonas aeruginosa dimethylarginine dimethylaminohydrolase, polymer, 261 residues, 29218 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pseudomonas aeruginosa dimethylarginine dimethylaminohydrolase: GAMDPEFMFKHIIARTPARS LVDGLTSSHLGKPDYAKALE QHNAYIEALQTCDVDITLLP PDERFPDSVFVEDPVLCTSR CAIITRPGAESRRGETEIIE ETVQHFYPGKVERIEAPGTV EAGDIMMVGDHFYIGESART NAEGARQMIAILEKHGLSGS VVRLEKVLHLKTGLAYLEHN NLLAAGEFVSKPEFQDFNII EIPEEESYAANCIWVNERVI MPAGYPRTREKIARLGYRVI EVDTSEYRKIDGGVSCMSLR F

Data sets:
Data typeCount
1H chemical shifts244
13C chemical shifts736
15N chemical shifts244

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PaDDAH protomer A1

Entities:

Entity 1, PaDDAH protomer A 261 residues - 29218 Da.

1   GLYALAMETASPPROGLUPHEMETPHELYS
2   HISILEILEALAARGTHRPROALAARGSER
3   LEUVALASPGLYLEUTHRSERSERHISLEU
4   GLYLYSPROASPTYRALALYSALALEUGLU
5   GLNHISASNALATYRILEGLUALALEUGLN
6   THRCYSASPVALASPILETHRLEULEUPRO
7   PROASPGLUARGPHEPROASPSERVALPHE
8   VALGLUASPPROVALLEUCYSTHRSERARG
9   CYSALAILEILETHRARGPROGLYALAGLU
10   SERARGARGGLYGLUTHRGLUILEILEGLU
11   GLUTHRVALGLNHISPHETYRPROGLYLYS
12   VALGLUARGILEGLUALAPROGLYTHRVAL
13   GLUALAGLYASPILEMETMETVALGLYASP
14   HISPHETYRILEGLYGLUSERALAARGTHR
15   ASNALAGLUGLYALAARGGLNMETILEALA
16   ILELEUGLULYSHISGLYLEUSERGLYSER
17   VALVALARGLEUGLULYSVALLEUHISLEU
18   LYSTHRGLYLEUALATYRLEUGLUHISASN
19   ASNLEULEUALAALAGLYGLUPHEVALSER
20   LYSPROGLUPHEGLNASPPHEASNILEILE
21   GLUILEPROGLUGLUGLUSERTYRALAALA
22   ASNCYSILETRPVALASNGLUARGVALILE
23   METPROALAGLYTYRPROARGTHRARGGLU
24   LYSILEALAARGLEUGLYTYRARGVALILE
25   GLUVALASPTHRSERGLUTYRARGLYSILE
26   ASPGLYGLYVALSERCYSMETSERLEUARG
27   PHE

Samples:

sample_1: Pseudomonas aeruginosa dimethylarginine dimethylaminohydrolase, [U-13C; U-15N; U-2H], 1.5 mM

Ex-cond_1: pH: 7.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CA)CBnot availablenot availablenot available
HN(COCA)CBnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 19615 19616 19742 19743 19744
PDB
DBJ BAK88928 BAP20511 BAP52091 GAA16731 GAJ52405
EMBL CAW28868 CCQ86504 CDH72250 CDH78521 CDI89341
GB AAG04584 ABJ10379 AEO76319 AFM66206 AGI82760
REF NP_249886 WP_003082466 WP_003086612 WP_003123128 WP_003133711
SP Q9I4E3
AlphaFold Q9I4E3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks