BMRB Entry 6063
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6063
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Liu, Y.; Liu, Z.; Androphy, E.; Chen, J.; Baleja, J.. "DESIGN AND CHARACTERIZATION OF HELICAL PEPTIDES THAT INHIBIT THE E6 PROTEIN OF
PAPILLOMAVIRUS" Biochemistry 43, 7421-7431 (2004).
PubMed: 15182185
Assembly members:
E6apc2, polymer, 35 residues, Formula weight is not available
Natural source: Common Name: Papillomavirus Taxonomy ID: 10558 Superkingdom: Viruses Kingdom: not available Genus/species: Papillomavirus not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
E6apc2: XYKFACPECPKRFMRSDHLS
KHITLHELLGEERRX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 165 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E6apc2 peptide | 1 |
Entities:
Entity 1, E6apc2 peptide 35 residues - Formula weight is not available
| 1 | ACE | TYR | LYS | PHE | ALA | CYS | PRO | GLU | CYS | PRO | ||||
| 2 | LYS | ARG | PHE | MET | ARG | SER | ASP | HIS | LEU | SER | ||||
| 3 | LYS | HIS | ILE | THR | LEU | HIS | GLU | LEU | LEU | GLY | ||||
| 4 | GLU | GLU | ARG | ARG | NH2 |
Samples:
sample_1: E6apc2 2.0 mM; imidazole, [U-2H], 10 mM; ZnSO4 4 mM; DTT 1 mM; H2O 90%; D2O 10%
sample_2: E6apc2 2.0 mM; imidazole, [U-2H], 10 mM; ZnSO4 4 mM; DTT 1 mM; D2O 99.96%
sample_cond_1: ionic strength: 4 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | not available | not available | sample_cond_1 |
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
Software:
xwinnmr v3.5 - collection, processing
CNS v1.1 - processing, refinement, structure solution
NMR spectrometers:
- Bruker AMX 500 MHz