BMRB Entry 6061

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6061

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the N-terminal 69 Amino Acid Residues of the ColE9 T1-61-DNase Fusion Protein
Deposition date:
2004-01-06
Original release date:
2004-10-29
Authors:
Tozawa, Kaeko; Macdonald, Colin; Collins, Emily; Penfold, Christopher; James, Richard; Kleanthous, Colin; Clayden, Nigel; Moore, Geoffrey
Citation:

Citation: Macdonald, Colin; Tozawa, Kaeko; Collins, Emily; Penfold, Christopher; James, Richard; Kleanthous, Colin; Clayden, Nigel; Moore, Geoffrey. "Characterisation of a mobile protein-binding epitope in the translocation domain of colicin E9 "  J. Biomol. NMR 30, 81-96 (2004).
PubMed: 15452437

Assembly members:

Assembly members:
T1-61-E9DNase fusion protein, polymer, 202 residues, 21292 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pNP330

Entity Sequences (FASTA):

Entity Sequences (FASTA):
T1-61-E9DNase fusion protein: MSGGDGRGHNTGAHSTSGNI NGGPTGIGVSGGASDGSGWS SENNPWGGGSGSGIHWGGGS GLVPRGSAMESKRNKPGKAT GKGKPVGDKWLDDAGKDSGA PIPDRIADKLRDKEFKSFDD FRKAVWEEVSKDPELSKNLN PSNKSSVSKGYSPFTPKNQQ VGGRKVYELHHDKPISQGGE VYDMDNIRVTTPKRHIDIHR GK

Data sets:
Data typeCount
1H chemical shifts268
13C chemical shifts169
15N chemical shifts60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1T1-61-DNase fusion protein1

Entities:

Entity 1, T1-61-DNase fusion protein 202 residues - 21292 Da.

1   METSERGLYGLYASPGLYARGGLYHISASN
2   THRGLYALAHISSERTHRSERGLYASNILE
3   ASNGLYGLYPROTHRGLYILEGLYVALSER
4   GLYGLYALASERASPGLYSERGLYTRPSER
5   SERGLUASNASNPROTRPGLYGLYGLYSER
6   GLYSERGLYILEHISTRPGLYGLYGLYSER
7   GLYLEUVALPROARGGLYSERALAMETGLU
8   SERLYSARGASNLYSPROGLYLYSALATHR
9   GLYLYSGLYLYSPROVALGLYASPLYSTRP
10   LEUASPASPALAGLYLYSASPSERGLYALA
11   PROILEPROASPARGILEALAASPLYSLEU
12   ARGASPLYSGLUPHELYSSERPHEASPASP
13   PHEARGLYSALAVALTRPGLUGLUVALSER
14   LYSASPPROGLULEUSERLYSASNLEUASN
15   PROSERASNLYSSERSERVALSERLYSGLY
16   TYRSERPROPHETHRPROLYSASNGLNGLN
17   VALGLYGLYARGLYSVALTYRGLULEUHIS
18   HISASPLYSPROILESERGLNGLYGLYGLU
19   VALTYRASPMETASPASNILEARGVALTHR
20   THRPROLYSARGHISILEASPILEHISARG
21   GLYLYS

Samples:

sample-1: T1-61-E9DNase fusion protein, [U-15N], 1.0 mM

sample-2: T1-61-E9DNase fusion protein, [U-13C; U-15N], 0.8 mM

cond_1: pH: 6.3; temperature: 288 K

cond_2: pH: 6.05; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
(HCA)CO(CA)NHnot availablenot availablenot available
HCCONHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HNNnot availablenot availablenot available

Software:

XEASY -

NMR spectrometers:

  • Varian INOVA 499.865 MHz
  • Varian INOVA 599.162 MHz

Related Database Links:

BMRB 4293
PDB
EMBL CAA33862
PRF 1615299E

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks