BMRB Entry 6059

Name: Ras-induced losing of interaction between C1 and RBD domains of Nore1
Published: 2008-07-01

Click here to enlarge.

PDB ID: 1rfh
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6059
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Ras-induced losing of interaction between C1 and RBD domains of Nore1

Deposition date:

2004-01-02

Original release date:

2008-07-01

Authors:

Guiberman, Elena; Wohlgemuth, Sabine; Herrmann, Christian; Harjes, Stefan; Muller, Karl-Heinz; Bayer, Peter

Citation:

Citation: Harjes, Elena; Harjes, Stefan; Wohlgemuth, Sabine; Muller, Karl-Heinz; Krieger, Elmar; Herrmann, Christian; Bayer, Peter. "GTP-Ras disrupts the intramolecular complex of C1 and RA domains of Nore1" Structure 14, 881-888 (2006).
PubMed: 16698549

Assembly members:

Assembly members:
putative Ras effector, polymer, 59 residues, 8255 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
putative Ras effector: PRVLAERGEGHRFVELALRG GPGWCDLCGREVLRQALRCA NCKFTCHSECRSLIQLDCR

Data sets:

assigned_chemical_shifts
- 1
coupling_constants
- J_values_set_1

Data type	Count
13C chemical shifts	110
1H chemical shifts	329
15N chemical shifts	55
coupling constants	42

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C1 domain, Nore1	1
2	ZINC ION (2+), 1	2
3	ZINC ION (2+), 2	2

Entities:

Entity 1, C1 domain, Nore1 59 residues - 8255 Da.

1

PRO

ARG

VAL

LEU

ALA

GLU

ARG

GLY

GLU

GLY

2

HIS

ARG

PHE

VAL

GLU

LEU

ALA

LEU

ARG

GLY

3

GLY

PRO

GLY

TRP

CYS

ASP

LEU

CYS

GLY

ARG

4

GLU

VAL

LEU

ARG

GLN

ALA

LEU

ARG

CYS

ALA

5

ASN

CYS

LYS

PHE

THR

CYS

HIS

SER

GLU

CYS

6

ARG

SER

LEU

ILE

GLN

LEU

ASP

CYS

ARG

Entity 2, ZINC ION (2+), 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: putative Ras effector, [U-95% 13C; U-90% 15N], 2.0 mM; pottasium phosphate puffer 20.0 mM; NaN3 0.5 mM; DTE 4 mM

condition_1: pH: 6.9; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCACB	sample_1	not available	condition_1
1H-15N NOESY	sample_1	not available	condition_1
CCONH	sample_1	not available	condition_1
HCCONH	sample_1	not available	condition_1
1H-N15 TOCSY	sample_1	not available	condition_1
HCCH-COSY	sample_1	not available	condition_1

Software:

Aurelia v2.8.11 - Visualisation of NMR-Spectra

NMR spectrometers:

INOVA Varian 600 MHz

PDB	1RFH 2FNF
DBJ	BAE33312
GB	AAC08580 AAH89605 EDL39713 EDL39714
REF	NP_061220
SP	Q5EBH1
AlphaFold	Q5EBH1