BMRB Entry 6057

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6057

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific resonance assignments of the tandem SH3 domains in autoinhibitory form of p47phox

Deposition date:

2003-12-27

Original release date:

2004-06-28

Authors:

Yuzawa, Satoru; Yokochi, Masashi; Fujioka, Yuko; Ogura, Kenji; Sumimoto, Hideki; Inagaki, Fuyuhiko

Citation:

Citation: Yuzawa, Satoru; Ogura, Kenji; Horiuchi, M.; Suzuki, N.; Fujioka, Yuko; Kataoka, M.; Sumimoto, Hideki; Inagaki, Fuyuhiko. "Solution structure of the tandem SH3 domains of p47phox in an autoinhibited form. " J. Biol. Chem. 29, 451-452 (2004).
PubMed: 15123602

Assembly members:

Assembly members:
p47phox, polymer, 193 residues, 21988.85 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPRO EX HTb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p47phox: GAMDITGPIILQTYRAIADY EKTSGSEMALSTGDVVEVVE KSESGWWFCQMKAKRGWIPA SFLEPLDSPDETEDPEPNYA GEPYVAIKAYTAVEGDEVSL LEGEAVEVIHKLLDGWWVIR KDDVTGYFPSMYLQKSGQDV SQAQRQIKRGAPPRRSSIRN AHSIHQRSRKRLSQDAYRRN SVRFLQQRRRQAR

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	551
1H chemical shifts	179
15N chemical shifts	179

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p47phox	1

Entities:

Entity 1, p47phox 193 residues - 21988.85 Da.

1

GLY

ALA

MET

ASP

ILE

THR

GLY

PRO

ILE

2

LEU

GLN

THR

TYR

ARG

ALA

ILE

ALA

ASP

TYR

3

GLU

LYS

THR

SER

GLY

SER

GLU

MET

ALA

LEU

4

SER

THR

GLY

ASP

VAL

GLU

VAL

GLU

5

LYS

SER

GLU

SER

GLY

TRP

PHE

CYS

GLN

6

MET

LYS

ALA

LYS

ARG

GLY

TRP

ILE

PRO

ALA

7

SER

PHE

LEU

GLU

PRO

LEU

ASP

SER

PRO

ASP

8

GLU

THR

GLU

ASP

PRO

GLU

PRO

ASN

TYR

ALA

9

GLY

GLU

PRO

TYR

VAL

ALA

ILE

LYS

ALA

TYR

10

THR

ALA

VAL

GLU

GLY

ASP

GLU

VAL

SER

LEU

11

LEU

GLU

GLY

GLU

ALA

VAL

GLU

VAL

ILE

HIS

12

LYS

LEU

ASP

GLY

TRP

VAL

ILE

ARG

13

LYS

ASP

VAL

THR

GLY

TYR

PHE

PRO

SER

14

MET

TYR

LEU

GLN

LYS

SER

GLY

GLN

ASP

VAL

15

SER

GLN

ALA

GLN

ARG

GLN

ILE

LYS

ARG

GLY

16

ALA

PRO

ARG

SER

ILE

ARG

ASN

17

ALA

HIS

SER

ILE

HIS

GLN

ARG

SER

ARG

LYS

18

ARG

LEU

SER

GLN

ASP

ALA

TYR

ARG

ASN

19

SER

VAL

ARG

PHE

LEU

GLN

ARG

20

GLN

ALA

ARG

Samples:

sample_1: p47phox, [U-90% 2H; U-98% 13C; U-98% 15N], 1.0 mM

Ex-cond_1: pH: 6.5; temperature: 298 K; ionic strength: 0.20 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	Ex-cond_1
HNCO	sample_1	not available	Ex-cond_1
HN(CA)CO	sample_1	not available	Ex-cond_1
HNCA	sample_1	not available	Ex-cond_1
HN(CO)CA	sample_1	not available	Ex-cond_1
NH(CO)CACB	sample_1	not available	Ex-cond_1
HNCACB	sample_1	not available	Ex-cond_1

Software:

nmrPipe - raw spectral data processing

Olivia - data processing, assignment analysis

NMR spectrometers:

Varian INOVAplus 600 MHz