BMRB Entry 6056

Title:
Yeast oligosaccharyltransferase subunit Ost4p   PubMed: 15001703
Deposition date:
2003-12-24
Original release date:
2004-05-15
Authors:
Zubkov, Sergey; Lennarz, William; Mohanty, Sergey
Citation:

Citation: Zubkov, Sergey; Lennarz, William; Mohanty, Smita. "Structural basis for the function of a minimembrane protein subunit of yeast oligosaccharyltransferase"  Proc. Natl. Acad. Sci. U. S. A. 101, 3821-3826 (2004).

Assembly members:

Assembly members:
yeast oligosaccharyltransferase subunit ost4p, polymer, 36 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
yeast oligosaccharyltransferase subunit ost4p: MISDEQLNSLAITFGIVMMT LIVIYHAVDSTMSPKN

Data sets:
Data typeCount
1H chemical shifts226

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ost4p1

Entities:

Entity 1, ost4p 36 residues - Formula weight is not available

1   METILESERASPGLUGLNLEUASNSERLEU
2   ALAILETHRPHEGLYILEVALMETMETTHR
3   LEUILEVALILETYRHISALAVALASPSER
4   THRMETSERPROLYSASN

Samples:

sample_1: yeast oligosaccharyltransferase subunit ost4p 1.0 mM

sample_cond_1: pH*: 7.0; temperature: 298 K; pressure: 1 atm

sample_cond_2: pH*: 7.0; temperature: 311 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablenot available
2D TOCSYsample_1not availablenot available
2D DQF-COSYsample_1not availablenot available

Software:

NMRPipe v2.2 - processing

NMRView v5.0.4 - data analysis

CYANA v1.0.6 - structure solution

ARIA v1.2 - refinement

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ GAA22023
EMBL CAA98811 CAY79054
GB AAB06797 AHY74787 EDN60132 EDV08487 EEU08972
REF NP_010049
SP Q99380
TPG DAA11634
AlphaFold Q99380