BMRB Entry 6050

Name: Solution structure of Sso7d mutant (K12L), NMR, 4 structures
Published: 2007-11-05

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6050

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of Sso7d mutant (K12L), NMR, 4 structures

Deposition date:

2003-12-16

Original release date:

2007-11-05

Authors:

Recca, T.; Consonni, R.; Alberti, E.; Arosio, I.; Zetta, L.

Citation:

Citation: Consonni, R.; Arosio, I.; Recca, T.; Fusi, P.; Zetta, L.. "Structural determinants responsible for the thermostability of Sso7d and its single point mutants" Proteins 67, 766-775 (2007).
PubMed: 17340638

Assembly members:

Assembly members:
SSO7D, polymer, 62 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SSO7D: ATVKFKYKGEELQVDISKIK KVWRVGKMISFTYDEGGGKT GRGAVSEKDAPKKLLQMLEK QK

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	446

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Endoribounuclease SSO7D	1

Entities:

Entity 1, Endoribounuclease SSO7D 62 residues - Formula weight is not available

1

ALA

THR

VAL

LYS

PHE

LYS

TYR

LYS

GLY

GLU

2

GLU

LEU

GLN

VAL

ASP

ILE

SER

LYS

ILE

LYS

3

LYS

VAL

TRP

ARG

VAL

GLY

LYS

MET

ILE

SER

4

PHE

THR

TYR

ASP

GLU

GLY

LYS

THR

5

GLY

ARG

GLY

ALA

VAL

SER

GLU

LYS

ASP

ALA

6

PRO

LYS

LEU

GLN

MET

LEU

GLU

LYS

7

GLN

LYS

Samples:

sample_1: SSO7D 2 mM; D2O 10%; H2O 90%

sample_2: SSO7D 2 mM; D2O 100%; H2O 90%

sample_cond_1: pH: 4.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
DQF-COSY	not available	not available	not available
2D TOCSY	not available	not available	not available
2D NOESY	not available	not available	not available

Software:

FELIX v98.0 - data analysis

DISCOVER v3.0 - refinement, structure solution

xwinnmr v2.6 - collection

NMR spectrometers:

Bruker DMX 500 MHz