BMRB Entry 6035

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6035

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Title:
1H, 13C and 15N assignments for human DNA ligase III 1-117
Deposition date:
2003-12-09
Original release date:
2004-10-29
Authors:
Kulczyk, Arkadiusz; Yang, Ji-Chun; Neuhaus, David
Citation:

Citation: Kulczyk, Arkadiusz; Yang, Ji-Chun; Neuhaus, David. "Solution structure and DNA binding of the zinc-finger domain from DNA ligase IIIalpha"  J. Mol. Biol. 341, 723-738 (2004).
PubMed: 15288782

Assembly members:

Assembly members:
DNA ligase III zinc finger, polymer, 117 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DNA ligase III zinc finger: MAEQRFCVDYAKRGTAGCKK CKEKIVKGVCRIGKVVPNPF SESGGDMKEWYHIKCMFEKL ERARATTKKIEDLTELEGWE ELEDNEKEQITQHIADLSSK AAGTPKKKAVVQAKLTT

Data sets:
Data typeCount
1H chemical shifts564
13C chemical shifts379
15N chemical shifts108

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DL3a 1-1171
2ZINC (II) ION2

Entities:

Entity 1, DL3a 1-117 117 residues - Formula weight is not available

1   METALAGLUGLNARGPHECYSVALASPTYR
2   ALALYSARGGLYTHRALAGLYCYSLYSLYS
3   CYSLYSGLULYSILEVALLYSGLYVALCYS
4   ARGILEGLYLYSVALVALPROASNPROPHE
5   SERGLUSERGLYGLYASPMETLYSGLUTRP
6   TYRHISILELYSCYSMETPHEGLULYSLEU
7   GLUARGALAARGALATHRTHRLYSLYSILE
8   GLUASPLEUTHRGLULEUGLUGLYTRPGLU
9   GLULEUGLUASPASNGLULYSGLUGLNILE
10   THRGLNHISILEALAASPLEUSERSERLYS
11   ALAALAGLYTHRPROLYSLYSLYSALAVAL
12   VALGLNALALYSLEUTHRTHR

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: DNA ligase III zinc finger, [U-98% 15N], 1 – 1.5 mM; ZINC (II) ION1 – 1.5 mM; sodium pyrophosphate 20 mM; 2H10-dithiothreitol 4 mM; zinc chloride 1 mM

sample_2: DNA ligase III zinc finger1 – 1.5 mM; ZINC (II) ION1 – 1.5 mM; sodium pyrophosphate 20 mM; 2H10 dithiothreitol 4 mM; zinc chloride 1 mM

Ex-cond_1: pH: 6.8; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYnot availablenot availableEx-cond_1
2D 1H-1H TOCSYnot availablenot availableEx-cond_1
2D 1H-1H 2QF COSYnot availablenot availableEx-cond_1
2D 15N-1H HSQCnot availablenot availableEx-cond_1
2D 13C-1H HSQCnot availablenot availableEx-cond_1
3D CBCANHnot availablenot availableEx-cond_1
3D CBCA(CO)NHnot availablenot availableEx-cond_1
3D HBHANHnot availablenot availableEx-cond_1
3D HBHA(CO)NHnot availablenot availableEx-cond_1
3D HCCH-TOCSYnot availablenot availableEx-cond_1
3D HCCH-COSYnot availablenot availableEx-cond_1
3D 15N NOESY-HSQCnot availablenot availableEx-cond_1
3D 15N HSQC-NOESY-HSQCnot availablenot availableEx-cond_1
3D 13C NOESY-HSQCnot availablenot availableEx-cond_1

Software:

xwinnmr v2.6 - acquiring, processing of data

Sparky v3.106 - analysing data

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 500 MHz

Related Database Links:

PDB
DBJ BAE25421 BAE29732 BAE39460
EMBL CAA59230
GB AAA85022 AAC53003 AAC53004 AAH09026 AAH49240
REF NP_001231032 NP_001252899 NP_001278174 NP_001278175 NP_001278176
SP P49916 P97386
AlphaFold P49916 P97386

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks