BMRB Entry 6034

Click here to enlarge.

PDB ID: 1qts
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6034
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 15N, 13C resonance assignments and 15N-1H residual dipolar couplings in 27 kDa alpha-adaptin ear-domain

Deposition date:

2003-12-05

Original release date:

2004-06-25

Authors:

Denisov, Alexei; Gehring, Kalle

Citation:

Citation: Denisov, Alexei; Ritter, Brigitte; McPherson, Peter; Gehring, Kalle. "Letter to the Editor: 1H, 15N, 13C resonance assignments and 15N-1H residual dipolar couplings for the alpha-adaptin ear-domain" J. Biomol. NMR 29, 441-442 (2004).
PubMed: 15213459

Assembly members:

Assembly members:
ear-domain of alpha-adaptin C, polymer, 238 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGET-2TK

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ear-domain of alpha-adaptin C: SEDNFARFVCKNNGVLFENQ LLQIGLKSEFRQNLGRMFIF YGNKTSTQFLNFTPTLICAD DLQTNLNLQTKPVDPTVDGG AQVQQVVNIECISDFTEAPV LNIQFRYGGTFQNVSVKLPI TLNKFFQPTEMASQDFFQRW KQLSNPQQEVQNIFKAKHPM DTEITKAKIIGFGSALLEEV DPNPANFVGAGIIHTKTTQI GCLLRLEPNLQAQMYRLTLR TSKDTVSQRLCELLSEQF

Data sets:

assigned_chemical_shifts
- shift_set_1
RDCs
- D_values_set_1

Data type	Count
13C chemical shifts	655
15N chemical shifts	207
1H chemical shifts	207
residual dipolar couplings	205

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	alpha-ear monomer	1

Entities:

Entity 1, alpha-ear monomer 238 residues - Formula weight is not available

1

SER

GLU

ASP

ASN

PHE

ALA

ARG

PHE

VAL

CYS

2

LYS

ASN

GLY

VAL

LEU

PHE

GLU

ASN

GLN

3

LEU

GLN

ILE

GLY

LEU

LYS

SER

GLU

PHE

4

ARG

GLN

ASN

LEU

GLY

ARG

MET

PHE

ILE

PHE

5

TYR

GLY

ASN

LYS

THR

SER

THR

GLN

PHE

LEU

6

ASN

PHE

THR

PRO

THR

LEU

ILE

CYS

ALA

ASP

7

ASP

LEU

GLN

THR

ASN

LEU

ASN

LEU

GLN

THR

8

LYS

PRO

VAL

ASP

PRO

THR

VAL

ASP

GLY

9

ALA

GLN

VAL

GLN

VAL

ASN

ILE

GLU

10

CYS

ILE

SER

ASP

PHE

THR

GLU

ALA

PRO

VAL

11

LEU

ASN

ILE

GLN

PHE

ARG

TYR

GLY

THR

12

PHE

GLN

ASN

VAL

SER

VAL

LYS

LEU

PRO

ILE

13

THR

LEU

ASN

LYS

PHE

GLN

PRO

THR

GLU

14

MET

ALA

SER

GLN

ASP

PHE

GLN

ARG

TRP

15

LYS

GLN

LEU

SER

ASN

PRO

GLN

GLU

VAL

16

GLN

ASN

ILE

PHE

LYS

ALA

LYS

HIS

PRO

MET

17

ASP

THR

GLU

ILE

THR

LYS

ALA

LYS

ILE

18

GLY

PHE

GLY

SER

ALA

LEU

GLU

VAL

19

ASP

PRO

ASN

PRO

ALA

ASN

PHE

VAL

GLY

ALA

20

GLY

ILE

HIS

THR

LYS

THR

GLN

ILE

21

GLY

CYS

LEU

ARG

LEU

GLU

PRO

ASN

LEU

22

GLN

ALA

GLN

MET

TYR

ARG

LEU

THR

LEU

ARG

23

THR

SER

LYS

ASP

THR

VAL

SER

GLN

ARG

LEU

24

CYS

GLU

LEU

SER

GLU

GLN

PHE

Samples:

sample_1: ear-domain of alpha-adaptin C, [U-99% 13C; U-99% 15N], 1.0 mM; sodium phosphate buffer 25 mM; NaCl 75 mM

sample_2: ear-domain of alpha-adaptin C, [U-99% 15N], 1.0 mM; sodium phosphate buffer 25 mM; NaCl 75 mM

sample_3: ear-domain of alpha-adaptin C, [U-99% 15N], 0.3 mM; sodium phosphate buffer 25 mM; NaCl 75 mM; Pf1 phage 2.5 mg/mL

Ex-cond_1: ionic strength: 0.10 M; pH: 7.3; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	not available	not available	not available
3D CBCA(CO)HN	not available	not available	not available
3D TROSY-HNCA	not available	not available	not available
3D TROSY-HN(CO)CA	not available	not available	not available
3D HNCO	not available	not available	not available
3D 15N-HSQC-NOESY	not available	not available	not available
2D IPAP 15N-HSQC	not available	not available	not available

Software:

xwinnmr v3.1 - collection, processing

XEASY v1.3.13 - data analysis

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1B9K 1KY6 1KY7 1KYD 1KYF 1KYU 1QTP 1QTS 1W80 2VJ0 3HS8
DBJ	BAC40392 BAD32332 BAE31240
EMBL	CAA33097 CAA37791
GB	AAB62703 AAH10597 AAH58099 AAH81786 EDL18116
REF	NP_031485 NP_112270 XP_003461349 XP_003509821 XP_003802841
SP	P17427 P18484
AlphaFold	P17427 P18484