BMRB Entry 6031

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6031

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N resonance assignments of human Notch-1 calcium binding EGF domains 11-13

Deposition date:

2003-12-03

Original release date:

2004-07-06

Authors:

Muranyi, Andreas; Hambleton, Sophie; Knott, Vroni; McMichael, Andrew; Handford, Penny; Downing, A. Kristina

Citation:

Citation: Muranyi, Andreas; Hambleton, Sophie; Knott, Vroni; McMichael, Andrew; Handford, Penny; Downing, A.. "Letter to the Editor: 1H, 13C, and 15N resonance assignments of human Notch-1 calcium binding EGF domains 11-13" J. Biomol. NMR 29, 443-444 (2004).
PubMed: 15213460

Assembly members:

Assembly members:
human Notch-1, polymer, 118 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo Sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human Notch-1: SAQDVDECSLGANPCEHAGK CINTLGSFECQCLQGYTGPR CEIDVNECVSNPCQNDATCL DQIGEFQCICMPGYEGVHCE VNTDECASSPCLHNGRCLDK INEFQCECPTGFTGHLCQ

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	382
15N chemical shifts	118
1H chemical shifts	661

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hN11-13	1
2	Calcium ion	2
3	Calcium ion	2
4	Calcium ion	2

Entities:

Entity 1, hN11-13 118 residues - Formula weight is not available

1

SER

ALA

GLN

ASP

VAL

ASP

GLU

CYS

SER

LEU

2

GLY

ALA

ASN

PRO

CYS

GLU

HIS

ALA

GLY

LYS

3

CYS

ILE

ASN

THR

LEU

GLY

SER

PHE

GLU

CYS

4

GLN

CYS

LEU

GLN

GLY

TYR

THR

GLY

PRO

ARG

5

CYS

GLU

ILE

ASP

VAL

ASN

GLU

CYS

VAL

SER

6

ASN

PRO

CYS

GLN

ASN

ASP

ALA

THR

CYS

LEU

7

ASP

GLN

ILE

GLY

GLU

PHE

GLN

CYS

ILE

CYS

8

MET

PRO

GLY

TYR

GLU

GLY

VAL

HIS

CYS

GLU

9

VAL

ASN

THR

ASP

GLU

CYS

ALA

SER

PRO

10

CYS

LEU

HIS

ASN

GLY

ARG

CYS

LEU

ASP

LYS

11

ILE

ASN

GLU

PHE

GLN

CYS

GLU

CYS

PRO

THR

12

GLY

PHE

THR

GLY

HIS

LEU

CYS

GLN

Entity 2, Calcium ion - Ca - 40.078 Da.

1

CA

Samples:

15N_sample: human Notch-1, [U-15N], 1.0 mM; CaCl2 15 mM; NaN3 0.02%

13C_15N_sample: human Notch-1, [U-13C; U-15N], 0.5 mM

Ex-cond_1: ionic strength: 0.09 M; pH: 6.1; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N TOCSY-HSQC	not available	not available	Ex-cond_1
1H-15N NOESY-HSQC	not available	not available	Ex-cond_1
HNCA	not available	not available	Ex-cond_1
HN(C0)CA	not available	not available	Ex-cond_1
HNCACB	not available	not available	Ex-cond_1
CBCA(CO)NH	not available	not available	Ex-cond_1
HNCO	not available	not available	Ex-cond_1
HCCH-TOCSY	not available	not available	Ex-cond_1
1H-13C NOESY	not available	not available	Ex-cond_1

Software:

FELIX v2.3 - Processing

NMRView v5.0 - Analysis

formatconverter v1.0 b2 - Format conversion to NMRStar

NMR spectrometers:

GE Omega 600 MHz
GE Omega 750 MHz