BMRB Entry 6025

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6025

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Title:
1H, 13C, 15N resonance assignments of the cytokine LECT2
Deposition date:
2003-11-28
Original release date:
2004-03-29
Authors:
Ito, Mie; Nagata, Koji; Yamagoe, Satoshi; Suzuki, Kazuo; Tanokura, Masaru
Citation:

Citation: Ito, Mie; Nagata, Koji; Yumoto, Fumiaki; Yamagoe, Satoshi; Suzuki, Kazuo; Adachi, Kyoko; Tanokura, Masaru. "Letter to the Editor: 1H, 13C, 15N resonance assignments of the cytokine LECT2"  J. Biomol. NMR 24, 543-544 (2004).

Assembly members:

Assembly members:
leukocyte cell-derived chemotaxin 2, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
leukocyte cell-derived chemotaxin 2: MHHHHHHGPWANICAGKSSN EIRTCDRHGCGQYSAQRSQR PHQGVDVLCSAGSTVYAPFT GMIVGQEKPYQNKNAINNGV RISGRGFCVKMFYIKPIKYK GPIKKGEKLGTLLPLQKVYP GIQSHVHIENCDSSDPTAYL

Data sets:
Data typeCount
13C chemical shifts334
15N chemical shifts115
1H chemical shifts215

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1LECT21

Entities:

Entity 1, LECT2 140 residues - Formula weight is not available

1   METHISHISHISHISHISHISGLYPROTRP
2   ALAASNILECYSALAGLYLYSSERSERASN
3   GLUILEARGTHRCYSASPARGHISGLYCYS
4   GLYGLNTYRSERALAGLNARGSERGLNARG
5   PROHISGLNGLYVALASPVALLEUCYSSER
6   ALAGLYSERTHRVALTYRALAPROPHETHR
7   GLYMETILEVALGLYGLNGLULYSPROTYR
8   GLNASNLYSASNALAILEASNASNGLYVAL
9   ARGILESERGLYARGGLYPHECYSVALLYS
10   METPHETYRILELYSPROILELYSTYRLYS
11   GLYPROILELYSLYSGLYGLULYSLEUGLY
12   THRLEULEUPROLEUGLNLYSVALTYRPRO
13   GLYILEGLNSERHISVALHISILEGLUASN
14   CYSASPSERSERASPPROTHRALATYRLEU

Samples:

sample_1: leukocyte cell-derived chemotaxin 2, [U-15N], 1.2 mM; glycerol 5%; Na2SO4 50 mM

sample_2: leukocyte cell-derived chemotaxin 2, [U-13C; U-15N], 0.8 mM; glycerol 5%; Na2SO4 50 mM

Ex-cond_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-1H-15N NOESYnot availablenot availablenot available
1H-1H-15N TOCSYnot availablenot availablenot available
1H-13C-1H DE-H(C)CH-TOCSYnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCANHnot availablenot availablenot available
HNCOnot availablenot availablenot available
(HCA)CO(CA)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available

Software:

NMRPipe v1.7 -

P-ROI v1.0 - Spectral analysis on Papers

SPARKY v3.76 -

CSI v1 - prediction secondary structure

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz

Related Database Links:

DBJ BAA23609 BAA25669 BAG36787 BAJ20851
GB AAB66905 AAC17734 AAH93670 AAI01580 AIC54696
REF NP_001181813 NP_002293 XP_002744226 XP_002815956 XP_003266460
SP O14960
AlphaFold O14960

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks