BMRB Entry 6019

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6019

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Title:
Solution structure of Archaeon DNA-binding protein ssh10b P62A mutant
Deposition date:
2003-11-21
Original release date:
2004-06-25
Authors:
Cui, Qiu; Tong, Yufeng; Wang, Jinfeng
Citation:

Citation: Fang, Xianyang; Cui, Qiu; Tong, Yufeng; Feng, Yingang; Shan, Lu; Huang, Li; Wang, Jinfeng. "A Stabilizing alpha/beta-Hydrophobic Core Greatly Contributes to Hyperthermostability of Archaeal [P62A]Ssh10b"  Biochemistry 47, 11212-11221 (2008).
PubMed: 18821773

Assembly members:

Assembly members:
P62A, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Sulfolobus shibatae   Taxonomy ID: 2286   Superkingdom: Archaea   Kingdom: not available   Genus/species: Sulfolobus shibatae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
P62A: MSSGTPTPSNVVLIGKKPVM NYVLAALTLLNQGVSEIVIK ARGRAISKAVDTVEIVRNRF LADKIEIKEIRVGSQVVTSQ DGRQSRVSTIEIAIRKK

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts92
1H chemical shifts603

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P62A subunit 11
2P62A subunit 21

Entities:

Entity 1, P62A subunit 1 97 residues - Formula weight is not available

1   METSERSERGLYTHRPROTHRPROSERASN
2   VALVALLEUILEGLYLYSLYSPROVALMET
3   ASNTYRVALLEUALAALALEUTHRLEULEU
4   ASNGLNGLYVALSERGLUILEVALILELYS
5   ALAARGGLYARGALAILESERLYSALAVAL
6   ASPTHRVALGLUILEVALARGASNARGPHE
7   LEUALAASPLYSILEGLUILELYSGLUILE
8   ARGVALGLYSERGLNVALVALTHRSERGLN
9   ASPGLYARGGLNSERARGVALSERTHRILE
10   GLUILEALAILEARGLYSLYS

Samples:

sample_1: P62A, [U-95% 13C; U-98% 15N], 1.5 mM

Ex-cond_1: pH: 5.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

Felix v98.0 - Data processing, Peak assignments

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 5226
PDB
EMBL CAA67066 CAC12668
GB AAK41236 ACP35357 ACP38017 ACP45513 ACP48689
REF NP_342446 WP_009992406 WP_010923153 YP_002829315 YP_002832002
SP P60848 P60849
AlphaFold P60848 P60849

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks