BMRB Entry 6016
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6016
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Liu, Dingjiang; Wang, Yu-sen; Gesell, Jennifer; Wilson, Eileen; Beyer, Brian; Wyss, Daniel. "Letter to the Editor: Backbone resonance assignments of the 45.3 kDa catalytic
domain of human BACE1" J. Biomol. NMR 29, 425-426 (2004).
Assembly members:
beta-site APP Cleaving Enzyme 1, polymer, 407 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
beta-site APP Cleaving Enzyme 1: DEEPEEPGRRGSFVEMVDNL
RGKSGQGYYVEMTVGSPPQT
LNILVDTGSSNFAVGAAPHP
FLHRYYQRQLSSTYRDLRKG
VYVPYTQGKWEGELGTDLVS
IPHGPNVTVRANIAAITESD
KFFINGSNWEGILGLAYAEI
ARPDDSLEPFFDSLVKQTHV
PNLFSLQLCGAGFPLNQSEV
LASVGGSMIIGGIDHSLYTG
SLWYTPIRREWYYEVIIVRV
EINGQDLKMDCKEYNYDKSI
VDSGTTNLRLPKKVFEAAVK
SIKAASSTEKFPDGFWLGEQ
LVCWQAGTTPWNIFPVISLY
LMGEVTNQSFRITILPQQYL
RPVEDVATSQDDCYKFAISQ
SSTGTVMGAVIMEGFYVVFD
RARKRIGFAVSACHVHDEFR
TAAVEGPFVTLDMEDCGYNI
PQTDEST
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 319 |
| 13C chemical shifts | 917 |
| 15N chemical shifts | 324 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BACE1 monomer | 1 |
Entities:
Entity 1, BACE1 monomer 407 residues - Formula weight is not available
| 1 | ASP | GLU | GLU | PRO | GLU | GLU | PRO | GLY | ARG | ARG | ||||
| 2 | GLY | SER | PHE | VAL | GLU | MET | VAL | ASP | ASN | LEU | ||||
| 3 | ARG | GLY | LYS | SER | GLY | GLN | GLY | TYR | TYR | VAL | ||||
| 4 | GLU | MET | THR | VAL | GLY | SER | PRO | PRO | GLN | THR | ||||
| 5 | LEU | ASN | ILE | LEU | VAL | ASP | THR | GLY | SER | SER | ||||
| 6 | ASN | PHE | ALA | VAL | GLY | ALA | ALA | PRO | HIS | PRO | ||||
| 7 | PHE | LEU | HIS | ARG | TYR | TYR | GLN | ARG | GLN | LEU | ||||
| 8 | SER | SER | THR | TYR | ARG | ASP | LEU | ARG | LYS | GLY | ||||
| 9 | VAL | TYR | VAL | PRO | TYR | THR | GLN | GLY | LYS | TRP | ||||
| 10 | GLU | GLY | GLU | LEU | GLY | THR | ASP | LEU | VAL | SER | ||||
| 11 | ILE | PRO | HIS | GLY | PRO | ASN | VAL | THR | VAL | ARG | ||||
| 12 | ALA | ASN | ILE | ALA | ALA | ILE | THR | GLU | SER | ASP | ||||
| 13 | LYS | PHE | PHE | ILE | ASN | GLY | SER | ASN | TRP | GLU | ||||
| 14 | GLY | ILE | LEU | GLY | LEU | ALA | TYR | ALA | GLU | ILE | ||||
| 15 | ALA | ARG | PRO | ASP | ASP | SER | LEU | GLU | PRO | PHE | ||||
| 16 | PHE | ASP | SER | LEU | VAL | LYS | GLN | THR | HIS | VAL | ||||
| 17 | PRO | ASN | LEU | PHE | SER | LEU | GLN | LEU | CYS | GLY | ||||
| 18 | ALA | GLY | PHE | PRO | LEU | ASN | GLN | SER | GLU | VAL | ||||
| 19 | LEU | ALA | SER | VAL | GLY | GLY | SER | MET | ILE | ILE | ||||
| 20 | GLY | GLY | ILE | ASP | HIS | SER | LEU | TYR | THR | GLY | ||||
| 21 | SER | LEU | TRP | TYR | THR | PRO | ILE | ARG | ARG | GLU | ||||
| 22 | TRP | TYR | TYR | GLU | VAL | ILE | ILE | VAL | ARG | VAL | ||||
| 23 | GLU | ILE | ASN | GLY | GLN | ASP | LEU | LYS | MET | ASP | ||||
| 24 | CYS | LYS | GLU | TYR | ASN | TYR | ASP | LYS | SER | ILE | ||||
| 25 | VAL | ASP | SER | GLY | THR | THR | ASN | LEU | ARG | LEU | ||||
| 26 | PRO | LYS | LYS | VAL | PHE | GLU | ALA | ALA | VAL | LYS | ||||
| 27 | SER | ILE | LYS | ALA | ALA | SER | SER | THR | GLU | LYS | ||||
| 28 | PHE | PRO | ASP | GLY | PHE | TRP | LEU | GLY | GLU | GLN | ||||
| 29 | LEU | VAL | CYS | TRP | GLN | ALA | GLY | THR | THR | PRO | ||||
| 30 | TRP | ASN | ILE | PHE | PRO | VAL | ILE | SER | LEU | TYR | ||||
| 31 | LEU | MET | GLY | GLU | VAL | THR | ASN | GLN | SER | PHE | ||||
| 32 | ARG | ILE | THR | ILE | LEU | PRO | GLN | GLN | TYR | LEU | ||||
| 33 | ARG | PRO | VAL | GLU | ASP | VAL | ALA | THR | SER | GLN | ||||
| 34 | ASP | ASP | CYS | TYR | LYS | PHE | ALA | ILE | SER | GLN | ||||
| 35 | SER | SER | THR | GLY | THR | VAL | MET | GLY | ALA | VAL | ||||
| 36 | ILE | MET | GLU | GLY | PHE | TYR | VAL | VAL | PHE | ASP | ||||
| 37 | ARG | ALA | ARG | LYS | ARG | ILE | GLY | PHE | ALA | VAL | ||||
| 38 | SER | ALA | CYS | HIS | VAL | HIS | ASP | GLU | PHE | ARG | ||||
| 39 | THR | ALA | ALA | VAL | GLU | GLY | PRO | PHE | VAL | THR | ||||
| 40 | LEU | ASP | MET | GLU | ASP | CYS | GLY | TYR | ASN | ILE | ||||
| 41 | PRO | GLN | THR | ASP | GLU | SER | THR |
Samples:
sample_1: beta-site APP Cleaving Enzyme 1, [U-2H; U-15N; U-13C], 0.9 mM
condi-1: pH: 7.5 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N TROSY | not available | not available | not available |
| 3D-TROSY-HNCACB | not available | not available | not available |
| 3D-TROSY-HNCACO | not available | not available | not available |
| 3D-TROSY-HNCA | not available | not available | not available |
| 3D-TROSY-HNCO | not available | not available | not available |
| 3D-TROSY-HNCOCA | not available | not available | not available |
| 3D-TROSY-CT-CACB(CO)NH | not available | not available | not available |
| 4D TROSY-HNCOCA | not available | not available | not available |
| 4D TROSY-HNCACO | not available | not available | not available |
Software:
NMRpipe - data processing
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA86463 BAB29317 BAB29370 BAC28156 BAC30889 |
| GB | AAF04142 AAF04143 AAF04144 AAF13715 AAF17079 |
| REF | NP_001039996 NP_001193977 NP_001247857 NP_001276783 NP_035922 |
| SP | P56817 P56818 P56819 Q2HJ40 |
| TPG | DAA22394 |
| AlphaFold | P56817 P56818 P56819 Q2HJ40 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks