BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6013

Title: 1H, 13C and 15N resonance assignments of the third spectrin repeat of alpha-actinin-4   PubMed: 15243185

Deposition date: 2003-11-20 Original release date: 2004-07-29

Authors: Kowalski, Kasper; Merkel, Anita; Booker, Grant

Citation: Kowalski, Kasper; Merkel, Anita; Booker, Grant. "Letter to the editor: 1H, 13C and 15N resonance assignments of the third spectrin repeat of alpha-actinin-4"  J. Biomol. NMR 29, 533-534 (2004).

Assembly members:
alpha-actinin-4 spectrin repeat 3, polymer, 129 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T2

Entity Sequences (FASTA):
alpha-actinin-4 spectrin repeat 3: GSTEKQLEAIDQLHLEYAKR AAPFNNWMESAMEDLQDMFI VHTIEEIEGLISAHDQFKST LPDADREREAILAIHKEAQR IAESNHIKLSGSNPYTTVTP QIINSKWEKVQQLVPKRDHA LLEEQSKQQ

Data sets:
Data typeCount
13C chemical shifts480
1H chemical shifts913
15N chemical shifts138

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-actinin-4 spectrin repeat 31

Entities:

Entity 1, alpha-actinin-4 spectrin repeat 3 129 residues - Formula weight is not available

1   GLYSERTHRGLULYSGLNLEUGLUALAILE
2   ASPGLNLEUHISLEUGLUTYRALALYSARG
3   ALAALAPROPHEASNASNTRPMETGLUSER
4   ALAMETGLUASPLEUGLNASPMETPHEILE
5   VALHISTHRILEGLUGLUILEGLUGLYLEU
6   ILESERALAHISASPGLNPHELYSSERTHR
7   LEUPROASPALAASPARGGLUARGGLUALA
8   ILELEUALAILEHISLYSGLUALAGLNARG
9   ILEALAGLUSERASNHISILELYSLEUSER
10   GLYSERASNPROTYRTHRTHRVALTHRPRO
11   GLNILEILEASNSERLYSTRPGLULYSVAL
12   GLNGLNLEUVALPROLYSARGASPHISALA
13   LEULEUGLUGLUGLNSERLYSGLNGLN

Samples:

sample_1: alpha-actinin-4 spectrin repeat 3 0.8 mM; NaH2PO4 10 mM; NaCl 100 mM; NaN3 0.01%

sample_2: alpha-actinin-4 spectrin repeat 3, [U-95% 15N], 0.6 mM; NaH2PO4 25 mM; NaCl 100 mM; NaN3 0.01%

sample_3: alpha-actinin-4 spectrin repeat 3, [U-95% 13C; U-95% 15N], 0.7 mM; NaH2PO4 25 mM; NaCl 100 mM; NaN3 0.01%

sample_conditions: pH: 6.75; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-TOCSYnot availablenot availablesample_conditions
1H-NOESYnot availablenot availablesample_conditions
15N/1H-HSQCnot availablenot availablesample_conditions
13C/1H-HSQCnot availablenot availablesample_conditions
15N NOESY-HSQCnot availablenot availablesample_conditions
HNHAnot availablenot availablesample_conditions
HNHBnot availablenot availablesample_conditions
HNCACBnot availablenot availablesample_conditions
CBCA(CO)NHnot availablenot availablesample_conditions
HNCOnot availablenot availablesample_conditions
C(CO)NH-TOCSYnot availablenot availablesample_conditions
H(CCO)NH-TOCSYnot availablenot availablesample_conditions

Software:

NMRPipe v1.1 - spectral processing

SPARKY v3.1 - analysis, assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA24447 BAE26366 BAE29264 BAJ20316
EMBL CAC10069 CAH90431
GB AAC53102 AAF20064 AAH05033 AAH13616 AAH15620
REF NP_001091521 NP_001127286 NP_001248731 NP_004915 NP_068695
SP A5D7D1 O43707 P57780 Q5RCS6 Q9QXQ0
TPG DAA19939

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts