BMRB Entry 6012

Name: Human Sgt1 binds to HSP90 through the CS domain and not the TPR domain
Published: 2004-05-15

Click here to enlarge.

PDB ID: 1rl1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6012
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Human Sgt1 binds to HSP90 through the CS domain and not the TPR domain

Deposition date:

2003-11-20

Original release date:

2004-05-15

Authors:

Lee, Young-Tae; Jacob, Jaison; Michowski, Wojciech; Nowotny, Marcin; Kuznicki, Jacek; Chazin, Walter

Citation:

Citation: Lee, Young-Tae; Jacob, Jaison; Michowski, Wojciech; Nowotny, Marcin; Kuznicki, Jacek; Chazin, Walter. "Human Sgt1 binds to HSP90 through the CS domain and not the TPR domain" J. Biol. Chem. 279, 16511-16517 (2004).
PubMed: 14761955

Assembly members:

Assembly members:
Sgt1 CS, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sgt1 CS: GSHMTHQSKIKYDWYQTESQ VVITLMIKNVQKNDVNVEFS EKELSALVKLPSGEDYNLKL ELLHPIIPEQSTFKVLSTKI EIKLKKPEAVRWEKLEGQGD VPTPKQFVADVKNL

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	497
1H chemical shifts	749
15N chemical shifts	115

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sgt1 CS	1

Entities:

Entity 1, Sgt1 CS 114 residues - Formula weight is not available

1

GLY

SER

HIS

MET

THR

HIS

GLN

SER

LYS

ILE

2

LYS

TYR

ASP

TRP

TYR

GLN

THR

GLU

SER

GLN

3

VAL

ILE

THR

LEU

MET

ILE

LYS

ASN

VAL

4

GLN

LYS

ASN

ASP

VAL

ASN

VAL

GLU

PHE

SER

5

GLU

LYS

GLU

LEU

SER

ALA

LEU

VAL

LYS

LEU

6

PRO

SER

GLY

GLU

ASP

TYR

ASN

LEU

LYS

LEU

7

GLU

LEU

HIS

PRO

ILE

PRO

GLU

GLN

8

SER

THR

PHE

LYS

VAL

LEU

SER

THR

LYS

ILE

9

GLU

ILE

LYS

LEU

LYS

PRO

GLU

ALA

VAL

10

ARG

TRP

GLU

LYS

LEU

GLU

GLY

GLN

GLY

ASP

11

VAL

PRO

THR

PRO

LYS

GLN

PHE

VAL

ALA

ASP

12

VAL

LYS

ASN

LEU

Samples:

sample_1: Sgt1 CS, [U-15N], 1 – 2 mM; H2O 93%; D2O 7%

sample_2: Sgt1 CS, [U-15N; U-13C], 1 – 2 mM; H2O 93%; D2O 7%

sample_3: Sgt1 CS, [U-13C], 1 – 2 mM; D2O 100%

sample_4: Sgt1 CS1 – 2 mM; D2O 100%

Ex-cond_1: pH: 4.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1-2 MM SGT1 CS DOMAIN U-15N, 20 MM ACETIC ACID, 5 MM DTT, 93% H2O, 7% D2O	not available	not available	Ex-cond_1
2D 15N-1H HSQC	not available	not available	Ex-cond_1
3D 15N-edited NOESY	not available	not available	Ex-cond_1
1-2 MM SGT1 CS DOMAIN U-15N,U-13C, 20 MM ACETIC ACID, 5 MM DTT, 93% H2O, 7% D2O	not available	not available	Ex-cond_1

Software:

nmrpipe - processing

sparky - assignment

pipp - structure-assisted NOESY analysis

NMR spectrometers:

Bruker Avance 600 MHz

PDB	1RL1
DBJ	BAF84091 BAF84817 BAG63688 BAI46473
EMBL	CAC51433
GB	AAD30062 AAH00911 AAP88800 AAQ01749 AAQ76039
REF	NP_001124384 NP_006695 XP_001084394 XP_001084516 XP_002742679
SP	Q9Y2Z0
AlphaFold	Q9Y2Z0