BMRB Entry 6005
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6005
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Hudson, Brian; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Recognition of the mRNA AU-Rich Element by the
Zinc Finger Domain of TIS11d" Nat. Struct. Biol. 11, 257-264 (2004).
PubMed: 14981510
Assembly members:
TIS11d TZF, polymer, 70 residues, 8298 Da.
5'-UUAUUUAUU-3', polymer, 7 residues, 2830 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
Entity Sequences (FASTA):
TIS11d TZF: STRYKTELCRPFEESGTCKY
GEKCQFAHGFHELRSLTRHP
KYKTELCRTFHTIGFCPYGP
RCHFIHNADE
5'-UUAUUUAUU-3': UUAUUUAUU
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 282 |
| 15N chemical shifts | 70 |
| 1H chemical shifts | 483 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TIS11d TZF | 1 |
| 2 | 5'-UUAUUUAUU-3' | 2 |
| 3 | ZINC (II) ION 1 | 3 |
| 4 | ZINC (II) ION 2 | 3 |
Entities:
Entity 1, TIS11d TZF 70 residues - 8298 Da.
| 1 | SER | THR | ARG | TYR | LYS | THR | GLU | LEU | CYS | ARG | |
| 2 | PRO | PHE | GLU | GLU | SER | GLY | THR | CYS | LYS | TYR | |
| 3 | GLY | GLU | LYS | CYS | GLN | PHE | ALA | HIS | GLY | PHE | |
| 4 | HIS | GLU | LEU | ARG | SER | LEU | THR | ARG | HIS | PRO | |
| 5 | LYS | TYR | LYS | THR | GLU | LEU | CYS | ARG | THR | PHE | |
| 6 | HIS | THR | ILE | GLY | PHE | CYS | PRO | TYR | GLY | PRO | |
| 7 | ARG | CYS | HIS | PHE | ILE | HIS | ASN | ALA | ASP | GLU |
Entity 2, 5'-UUAUUUAUU-3' 7 residues - 2830 Da.
| 1 | U | U | A | U | U | U | A | U | U |
Entity 3, ZINC (II) ION 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: TIS11d TZF, [U-15N], 0.5 mM; 5'-UUAUUUAUU-3' 0.5 mM; TrisHCl 10 mM; KCl 20 mM; DTT 2.5 mM; ZnSO4 25 uM; H20 95%; D2O 5%
sample_2: TIS11d TZF, [U-13C; U-15N], 0.5 mM; 5'-UUAUUUAUU-3' 0.5 mM; TrisHCl 10 mM; KCl 20 mM; DTT 2.5 mM; ZnSO4 25 uM; H20 95%; D2O 5%
sample_3: TIS11d TZF, [U-13C; U-15N], 0.5 mM; 5'-UUAUUUAUU-3' 0.5 mM; Tris-HCl 10 mM; KCl 20 mM; DTT 2.5 mM; ZnSO4 25 uM; D2O 100%
sample_4: TIS11d TZF, [U-13C; U-15N], 0.5 mM; 5'-UUAUUUAUU-3' 0.5 mM; TrisHCl 10 mM; KCl 20 mM; DTT 2.5 mM; ZnSO4 25 uM; Pf1 filamentous phage 12 mg/mL; D2O 100%
Conditions_1: ionic strength: 0.02 M; pH: 6.2; temperature: 298 K
Conditions_2: ionic strength: 0.02 M; pH: 6.2; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-15N-HMQC | not available | not available | not available |
| 1H-13C-HSQC | not available | not available | not available |
| 1H-13C-CT-HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNCO | not available | not available | not available |
| C(CO)NH-TOCSY | not available | not available | not available |
| H(CCO)NH-TOCSY | not available | not available | not available |
| CCH-COSY | not available | not available | not available |
| CCH-TOCSY | not available | not available | not available |
| H(C)CH-COSY | not available | not available | not available |
| H(C)CH-TOCSY | not available | not available | not available |
| (HB)CB(CGCD)HD | not available | not available | not available |
| (HB)CB(CGCDCE)HE | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 13C F1-edited, F3-separated NOESY | not available | not available | not available |
| 2D 13C double-half-filtered NOESY | not available | not available | not available |
| 2D 13C double-half-filtered TOCSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| HACAHB-COSY | not available | not available | not available |
| 13C-{13CO} spin-echo difference CT-HSQC | not available | not available | not available |
| 13C-{15N} spin-echo difference CT-HSQC | not available | not available | not available |
| IPAP-[1H,15N]-HSQC | not available | not available | not available |
| IPAP-HNCO | not available | not available | not available |
Software:
xwinnmr v2.1 - data collection
NMRPipe v2.1 - data processing
NMRView v5.0 - data analysis
SANE vn/a - automatic NOE assignment
DYANA v1.5 - structure determination
AMBER v8.0 - structure refinement
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker DMX 750 MHz
- Bruker Avance 900 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks