BMRB Entry 6004

Name: 1H, 13C, 15N assignments of human Cofilin
Published: 2004-11-15

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PDB ID: 1q8g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6004
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N assignments of human Cofilin

Deposition date:

2003-11-14

Original release date:

2004-11-15

Authors:

Zierler-Gould, K.; Pope, B.; Weeds, A.; Ball, L.

Citation:

Citation: Zierler-Gould, K.; Pope, B.; Weeds, A.; Ball, L.. "Letter to the editor: Backbone and sidechain 1H, 13C and 15N resonance assignments of human cofilin " J. Biomol. NMR 29, 429-430 (2004).
PubMed: 15213453

Assembly members:

Assembly members:
cofilin, polymer, 166 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cofilin: MASGVAVSDGVIKVFNDMKV RKSSTPEEVKKRKKAVLFCL SEDKKNIILEEGKEILVGDV GQTVDDPYATFVKMLPDKDC RYALYDATYETKESKKEDLV FIFWAPESAPLKSKMIYASS KDAIKKKLTGIKHELQANCY EEVKDRCTLAEKLGGSAVIS LEGKPL

Data sets:

assigned_chemical_shifts
- cofilin_chemical_shifts

Data type	Count
1H chemical shifts	1166
13C chemical shifts	534
15N chemical shifts	164

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cofilin	1

Entities:

Entity 1, cofilin 166 residues - Formula weight is not available

1

MET

ALA

SER

GLY

VAL

ALA

VAL

SER

ASP

GLY

2

VAL

ILE

LYS

VAL

PHE

ASN

ASP

MET

LYS

VAL

3

ARG

LYS

SER

THR

PRO

GLU

VAL

LYS

4

LYS

ARG

LYS

ALA

VAL

LEU

PHE

CYS

LEU

5

SER

GLU

ASP

LYS

ASN

ILE

LEU

GLU

6

GLU

GLY

LYS

GLU

ILE

LEU

VAL

GLY

ASP

VAL

7

GLY

GLN

THR

VAL

ASP

PRO

TYR

ALA

THR

8

PHE

VAL

LYS

MET

LEU

PRO

ASP

LYS

ASP

CYS

9

ARG

TYR

ALA

LEU

TYR

ASP

ALA

THR

TYR

GLU

10

THR

LYS

GLU

SER

LYS

GLU

ASP

LEU

VAL

11

PHE

ILE

PHE

TRP

ALA

PRO

GLU

SER

ALA

PRO

12

LEU

LYS

SER

LYS

MET

ILE

TYR

ALA

SER

13

LYS

ASP

ALA

ILE

LYS

LEU

THR

GLY

14

ILE

LYS

HIS

GLU

LEU

GLN

ALA

ASN

CYS

TYR

15

GLU

VAL

LYS

ASP

ARG

CYS

THR

LEU

ALA

16

GLU

LYS

LEU

GLY

SER

ALA

VAL

ILE

SER

17

LEU

GLU

GLY

LYS

PRO

LEU

Samples:

sample_1: cofilin, [U-15N; U-13C], 0.8 mM; phosphate buffer 10 mM; H2O 90%; D2O 10%

sample_2: cofilin, [U-15N], 1 mM; phosphate buffer 10 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0; temperature: 300 K; ionic strength: 10 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY-HSQC in H2O (aliphatic centred)	not available	not available	not available
3D 13C-separated NOESY-HSQC in H2O (aromatic centred)	not available	not available	not available
3D 15N-separated NOESY-HSQC	not available	not available	not available
3D HNHA	not available	not available	not available
3D HNHB	not available	not available	not available
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
2D DQF-COSY	not available	not available	not available

Software:

XWINNMR v1.3 - acquisition, processing

AZARA v2.1 - processing, viewing

ANSIG v3.3 - assignment, integration, creation of NOE restraint lists

CNS v1.1 - structure solution, refinement

NMR spectrometers:

Bruker DMX 750 MHz

PDB	1Q8G 1Q8X 3J0S 4BEX
DBJ	BAA00364 BAA00589 BAB29074 BAB32114 BAC34363
EMBL	CAA44694 CAA64685
GB	AAA31020 AAA64501 AAH11005 AAH12265 AAH12318
REF	NP_001004043 NP_001009484 NP_001015655 NP_001253534 NP_005498
SP	P10668 P18760 P23528 P45592 Q4R5C0
TPG	DAA13572
AlphaFold	P10668 P18760 P23528 P45592 Q4R5C0