BMRB Entry 6003

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR6003

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Complete Resonance Assignments of Bundlin (BfpA) from the Bundle- forming Pilus of Enteropathogenic Escherichia coli

Deposition date:

2003-11-11

Original release date:

2004-07-06

Authors:

Ramboarina, Stephanie; Fernandes, Paula; Simpson, Peter; Frankel, Gad; Donnenberg, Michael; Matthews, Steve

Citation:

Citation: Ramboarina, Stephanie; Fernandes, Paula; Simpson, Peter; Frankel, Gad; Donnenberg, Michael; Matthews, Stephen. "Letter to the Editor: Complete Resonance Assignments of Bundlin (BfpA) from the Bundle-forming Pilus of Enteropathogenic Escherichia coli" J. Biomol. NMR 29, 427-428 (2004).
PubMed: 15213452

Assembly members:

Assembly members:
bundlin, polymer, 164 residues, 18300 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET39b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bundlin: HHHHHHMEQSASDSNKSQNA ISEVMSATSAINGLYIGQTS YSGLDSTILLNTSAIPDNYK DTTNKKITNPFGGELNVGPA NNNTAFGYYLTLTRLDKAAC VSLATLNLGTSAKGYGVNIS GENNITSFGNSADQAAKSTA ITPAEAATACKNTDSTNKVT YFMK

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	938
13C chemical shifts	610
15N chemical shifts	148

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	bfpA monomer	1

Entities:

Entity 1, bfpA monomer 164 residues - 18300 Da.

1

HIS

MET

GLU

GLN

SER

2

ALA

SER

ASP

SER

ASN

LYS

SER

GLN

ASN

ALA

3

ILE

SER

GLU

VAL

MET

SER

ALA

THR

SER

ALA

4

ILE

ASN

GLY

LEU

TYR

ILE

GLY

GLN

THR

SER

5

TYR

SER

GLY

LEU

ASP

SER

THR

ILE

LEU

6

ASN

THR

SER

ALA

ILE

PRO

ASP

ASN

TYR

LYS

7

ASP

THR

ASN

LYS

ILE

THR

ASN

PRO

8

PHE

GLY

GLU

LEU

ASN

VAL

GLY

PRO

ALA

9

ASN

THR

ALA

PHE

GLY

TYR

LEU

10

THR

LEU

THR

ARG

LEU

ASP

LYS

ALA

CYS

11

VAL

SER

LEU

ALA

THR

LEU

ASN

LEU

GLY

THR

12

SER

ALA

LYS

GLY

TYR

GLY

VAL

ASN

ILE

SER

13

GLY

GLU

ASN

ILE

THR

SER

PHE

GLY

ASN

14

SER

ALA

ASP

GLN

ALA

LYS

SER

THR

ALA

15

ILE

THR

PRO

ALA

GLU

ALA

THR

ALA

CYS

16

LYS

ASN

THR

ASP

SER

THR

ASN

LYS

VAL

THR

17

TYR

PHE

MET

LYS

Samples:

sample_1: bundlin, [U-95% 13C; U-95% 15N], 1 mM

Ex-cond_1: pH: 5.2 na; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-13C HSQC	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HNCO	not available	not available	not available
HBHA(CO)NH	not available	not available	not available
H(C)CH-TOCSY	not available	not available	not available
(H)CC(CO)NH-TOCSY	not available	not available	not available
15N NOESY-HSQC	not available	not available	not available
13C NOESY-HSQC	not available	not available	not available

Software:

NMRView v5.0.4 -

NMR spectrometers:

Bruker DRX 500 MHz
Varian INOVA 800 MHz

PDB	1ZWT
DBJ	BAA84838 BAE98279 BAE98284 BAE98287 BAE98291
EMBL	CAA78167 CAA92326 CAJ34994 CAS07439 CAY54182
GB	AAC44040 AAG16257 AAG16261 AAG16262 AAG16265
REF	NP_053065 WP_000253757 WP_000253758 YP_001965379 YP_002332157
SP	P33553 P58997
AlphaFold	P33553 P58997