BMRB Entry 5997
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5997
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gaponenko, Vadim; Sarma, Siddhartha; Altieri, Amanda; Horita, David; Li, Jess; Byrd, R.. "Improving the Accuracy of NMR Structures of Large Proteins Using Pseudocontact
Shifts as Long-range Restraints" J. Biomol. NMR 28, 205-212 (2004).
Assembly members:
STAT4 N-terminal domain, polymer, 123 residues, 29500 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
STAT4 N-terminal domain: SQWNQVQQLEIKFLEQVDQF
YDDNFPMEIRHLLAQWIENQ
DWEAASNNETMATILLQNLL
IQLDEQLGRVSKEKNLLLIH
NLKRIRKVLQGKFHGNPMHV
AVVISNCLREERRILAAANM
PVQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 304 |
| 15N chemical shifts | 119 |
| 1H chemical shifts | 119 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | STAT4NT subunit 1 | 1 |
| 2 | STAT4NT subunit 2 | 1 |
Entities:
Entity 1, STAT4NT subunit 1 123 residues - 29500 Da.
| 1 | SER | GLN | TRP | ASN | GLN | VAL | GLN | GLN | LEU | GLU | ||||
| 2 | ILE | LYS | PHE | LEU | GLU | GLN | VAL | ASP | GLN | PHE | ||||
| 3 | TYR | ASP | ASP | ASN | PHE | PRO | MET | GLU | ILE | ARG | ||||
| 4 | HIS | LEU | LEU | ALA | GLN | TRP | ILE | GLU | ASN | GLN | ||||
| 5 | ASP | TRP | GLU | ALA | ALA | SER | ASN | ASN | GLU | THR | ||||
| 6 | MET | ALA | THR | ILE | LEU | LEU | GLN | ASN | LEU | LEU | ||||
| 7 | ILE | GLN | LEU | ASP | GLU | GLN | LEU | GLY | ARG | VAL | ||||
| 8 | SER | LYS | GLU | LYS | ASN | LEU | LEU | LEU | ILE | HIS | ||||
| 9 | ASN | LEU | LYS | ARG | ILE | ARG | LYS | VAL | LEU | GLN | ||||
| 10 | GLY | LYS | PHE | HIS | GLY | ASN | PRO | MET | HIS | VAL | ||||
| 11 | ALA | VAL | VAL | ILE | SER | ASN | CYS | LEU | ARG | GLU | ||||
| 12 | GLU | ARG | ARG | ILE | LEU | ALA | ALA | ALA | ASN | MET | ||||
| 13 | PRO | VAL | GLN |
Samples:
sample_1: STAT4 N-terminal domain, [U-15N; U-13C], 0.8 mM
cond_1: pH: 5.3; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| H(C)(CO)NH | not available | not available | not available |
| (H)C(CO)NH | not available | not available | not available |
| 15N/15N HSQC-NOESY-HSQC | not available | not available | not available |
| 13C/15N HMQC-NOESY-HSQC | not available | not available | not available |
| 13C/13C HMQC-NOESY-HSQC | not available | not available | not available |
| 13C/13C HSQC-NOESY-HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HN(CA)CB | not available | not available | not available |
| HN(COCA)CB | not available | not available | not available |
Software:
ANSIG v3.3 - peak assignment
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAE21295 BAE33957 BAE87129 BAG61799 BAG62516 |
| GB | AAA19453 AAA19692 AAB05605 AAC12758 AAH31212 |
| REF | NP_001012226 NP_001077161 NP_001184234 NP_001230764 NP_003142 |
| SP | P42228 Q14765 |
| TPG | DAA32590 |
| AlphaFold | P42228 Q14765 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks