BMRB Entry 5992

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5992
MolProbity Validation Chart

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Title:
1H, 13C and 15N resonance assignment and secondary structure of the C-terminal domain of the human centrin 2 in complex with a 17 residue peptide from Xeroderma Pigmentosum group C protein
Deposition date:
2003-11-03
Original release date:
2004-05-15
Authors:
Miron, Simona; Yang, Ao; Blouquit, Yves; Duchambon, Patricia; Craescu, Constantin
Citation:

Citation: Miron, Simona; Yang, Ao; Blouquit, Yves; Duchambon, Patricia; Craescu, Constantin. "1H, 13C and 15N resonance assignment and secondary structure of the C-terminal domain of the human centrin 2 in complex with a 17 residue peptide from Xeroderma Pigmentosum group C protein"  J. Biol. Chem. 278, 40252-40261 (2003).
PubMed: 12890685

Assembly members:

Assembly members:
C-terminal domain of human centrin 2, polymer, 79 residues, Formula weight is not available
P1-XPC peptide, polymer, 17 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal domain of human centrin 2: TQKMSEKDTKEEILKAFKLF DDDETGKISFKNLKRVAKEL GENLTDEELQEMIDEADRDG DGEVSEQEFLRIMKKTSLY
P1-XPC peptide: NWKLLAKGLLIRERLKR

Data typeCount
1H chemical shifts619
13C chemical shifts247
15N chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1C-terminal domain of human centrin 21
2P1-XPC Peptide2
3Calcium ion3

Entities:

Entity 1, C-terminal domain of human centrin 2 79 residues - Formula weight is not available

1   THRGLNLYSMETSERGLULYSASPTHRLYS
2   GLUGLUILELEULYSALAPHELYSLEUPHE
3   ASPASPASPGLUTHRGLYLYSILESERPHE
4   LYSASNLEULYSARGVALALALYSGLULEU
5   GLYGLUASNLEUTHRASPGLUGLULEUGLN
6   GLUMETILEASPGLUALAASPARGASPGLY
7   ASPGLYGLUVALSERGLUGLNGLUPHELEU
8   ARGILEMETLYSLYSTHRSERLEUTYR

Entity 2, P1-XPC Peptide 17 residues - Formula weight is not available

1   ASNTRPLYSLEULEUALALYSGLYLEULEU
2   ILEARGGLUARGLEULYSARG

Entity 3, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_one: C-terminal domain of human centrin 2 1.2 mM; P1-XPC peptide 1.2 mM; H2O 93%; D2O 7%

sample_two: C-terminal domain of human centrin 2, [U-15N], 1.2 mM; P1-XPC peptide, [U-15N], 1.2 mM; H2O 93%; D2O 7%

sample_three: C-terminal domain of human centrin 2, [U-13C; U-15N], 1.2 mM; P1-XPC peptide, [U-13C; U-15N], 1.2 mM; H2O 93%; D2O 7%

Sample_conditions_one: pH: 6.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H COSYnot availablenot availableSample_conditions_one
TOCSYnot availablenot availableSample_conditions_one
NOESYnot availablenot availableSample_conditions_one
1H-15N 2D HSQCnot availablenot availableSample_conditions_one
3D NOESY-HSQCnot availablenot availableSample_conditions_one
3D TOCSY-HSQCnot availablenot availableSample_conditions_one
3D HNCAnot availablenot availableSample_conditions_one
HN(CO)CAnot availablenot availableSample_conditions_one
HCCH-TOCSYnot availablenot availableSample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

PDB
DBJ BAA04651 BAD96564 BAF82450 BAG58555 BAG58555 BAF82450 BAD96564 BAA04651
EMBL CAA46158 CAH90958 CAH90958 CAA46158
GB AAF87574 AAH16620 AAM77801 ADI50189 AHW56602
PRF 1817398A 1817398A
REF NP_004619 XP_001091593 XP_001091951 XP_001092063 XP_002798747 XP_001092063 XP_001091951 XP_001091827 NP_004619
SP Q01831
SWISS-PROT Q01831
GenBank EAW64189 EAW64188 AAM77801 AAH16620 AAF87574
AlphaFold Q01831

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks