BMRB Entry 5989

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5989
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Cytotoxin I from the venom of the Central Asian cobra Naja Oxiana, in aqueous solution
Deposition date:
2003-10-31
Original release date:
2005-05-20
Authors:
Dubinnyi, Maxim
Citation:

Citation: Dubovskii, P.; Lesovoy, D.; Dubinnyi, Maxim; Konshina, A.; Utkin, Yuri; Efremov, R.; Arseniev, Alexander. "Interaction of three-finger toxins with phospholipid membranes: comparison of S- and P-type cytotoxins"  Biochem. J. 387, 807-815 (2005).
PubMed: 15584897

Assembly members:

Assembly members:
Cytotocin I, polymer, 60 residues, 6821 Da.

Natural source:

Natural source:   Common Name: Central Asian Cobra   Taxonomy ID: 8657   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Naja Naja oxiana

Experimental source:

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cytotocin I: LKCNKLVPIAYKTCPEGKNL CYKMFMMSDLTIPVKRGCID VCPKNSLLVKYVCCNTDRCN

Data sets:
Data typeCount
1H chemical shifts842
coupling constants107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CTI major form1
2CTI minor form1

Entities:

Entity 1, CTI major form 60 residues - 6821 Da.

1   LEULYSCYSASNLYSLEUVALPROILEALA
2   TYRLYSTHRCYSPROGLUGLYLYSASNLEU
3   CYSTYRLYSMETPHEMETMETSERASPLEU
4   THRILEPROVALLYSARGGLYCYSILEASP
5   VALCYSPROLYSASNSERLEULEUVALLYS
6   TYRVALCYSCYSASNTHRASPARGCYSASN

Samples:

sample_1: Cytotocin I 3.0 mM; KCl 0.05 M

condition_1: pH: 6.0; temperature: 303 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
DQF-COSYsample_1not availablecondition_1
TOCSYsample_1not availablecondition_1
NOESYsample_1not availablecondition_1

Software:

XWINNMR v3.1a - spectral data processing

XEASY v1.2.11 - peak picking, sequence-specific assignment, manual NOE assignment

CYANA v1.0.6 - automated NOE assignment, structure calculation

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAB24495
PRF 763620A
SP P01451
AlphaFold P01451