BMRB Entry 5983

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5983

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Title:
1H, 13C and 15N resonance assignments of human 5-10-methenyltetrahydrofolate synthetase
Deposition date:
2003-10-24
Original release date:
2004-07-28
Authors:
Copeland, Evelyne; Ekiel, Irena; Cygler, Miroslaw
Citation:

Citation: Copeland, Evelyne; Ekiel, Irena; Cygler, Miroslaw. "Letter to the Editor: 1H, 13C and 15N resonance assignments of human 5,10-methenyltetrahydrofolate synthetase"  J. Biomol. NMR 29, 547-548 (2004).
PubMed: 15243192

Assembly members:

Assembly members:
5,10-formyltetrahydrofolate synthetase, polymer, 202 residues, 23124 Da.
FON, non-polymer, 473.439 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 11c

Entity Sequences (FASTA):

Entity Sequences (FASTA):
5,10-formyltetrahydrofolate synthetase: AAAAVSSAKRSLRGELKQRL RAMSAEERLRQSRVLSQKVI AHSEYQKSKRISIFLSMQDE IETEEIIKDIFQRGKICFIP RYRFQSNHMDMVRIESPEEI SLLPKTSWNIPQPGEGDVRE EALSTGGLDLIFMPGLGFDK HGNRLGRGKGYYDAYLKRCL QHQEVKPYTLALAFKEQICL QVPVNENDMKVDEVLYEDSS TA

Data sets:
Data typeCount
13C chemical shifts739
15N chemical shifts187
1H chemical shifts984

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MTHFS monomer1
25-formyltetrahydrofolate2

Entities:

Entity 1, MTHFS monomer 202 residues - 23124 Da.

1   ALAALAALAALAVALSERSERALALYSARG
2   SERLEUARGGLYGLULEULYSGLNARGLEU
3   ARGALAMETSERALAGLUGLUARGLEUARG
4   GLNSERARGVALLEUSERGLNLYSVALILE
5   ALAHISSERGLUTYRGLNLYSSERLYSARG
6   ILESERILEPHELEUSERMETGLNASPGLU
7   ILEGLUTHRGLUGLUILEILELYSASPILE
8   PHEGLNARGGLYLYSILECYSPHEILEPRO
9   ARGTYRARGPHEGLNSERASNHISMETASP
10   METVALARGILEGLUSERPROGLUGLUILE
11   SERLEULEUPROLYSTHRSERTRPASNILE
12   PROGLNPROGLYGLUGLYASPVALARGGLU
13   GLUALALEUSERTHRGLYGLYLEUASPLEU
14   ILEPHEMETPROGLYLEUGLYPHEASPLYS
15   HISGLYASNARGLEUGLYARGGLYLYSGLY
16   TYRTYRASPALATYRLEULYSARGCYSLEU
17   GLNHISGLNGLUVALLYSPROTYRTHRLEU
18   ALALEUALAPHELYSGLUGLNILECYSLEU
19   GLNVALPROVALASNGLUASNASPMETLYS
20   VALASPGLUVALLEUTYRGLUASPSERSER
21   THRALA

Entity 2, 5-formyltetrahydrofolate - C20 H23 N7 O7 - 473.439 Da.

1   FON

Samples:

sample_1: 5,10-formyltetrahydrofolate synthetase, [U-15N], 1.5 mM; 5-FORMYL-TETRAHYDROFOLIC ACID 1.5 mM; KCl 100 mM

sample_2: 5,10-formyltetrahydrofolate synthetase, [U-13C; U-15N], 1.5 mM; 5-FORMYL-TETRAHYDROFOLIC ACID 1.5 mM; KCl 100 mM

Ex-cond_1: ionic strength: 0.15 M; pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
HN(CA)COnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
NOESY-HSQCnot availablenot availableEx-cond_1
HNHAnot availablenot availableEx-cond_1
HCCH-COSYnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1

Software:

GIFA v4.3 -

NMRPipe v2.1 -

XEASY v1.3.13 -

NMRView v5.0.4 -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 800 MHz
  • Varian UNITY-plus 800 MHz

Related Database Links:

PDB
DBJ BAI45876
GB AAC41945 AAH12417 AAH19921 AIC55813 EAW99132
REF NP_001186687 NP_006432 XP_001154837 XP_001154891 XP_003275449
SP P49914
AlphaFold P49914

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks