BMRB Entry 5975

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PDB ID: 1r2u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5975
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N chemical shift assignments for the N-domain of trout cardiac troponin C at 30 and 7 C

Deposition date:

2003-10-16

Original release date:

2003-12-08

Authors:

Blumenschein, Tharin; Gillis, Todd; Tibbits, Glen; Sykes, Brian

Citation:

Citation: Gillis, Todd; Blumenschein, Tharin; Sykes, Brian; Tibbits, Glen. "Effect of temperature and the F27W mutation on the Ca2+ activated structural transition of trout cardiac troponin C" Biochemistry 42, 6418-6426 (2003).
PubMed: 12767223

Assembly members:

Assembly members:
trout troponin C, polymer, 89 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: rainbow trout Taxonomy ID: 8022 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Oncorhynchus mykiss

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
trout troponin C: MNDIYKAAVEQLTDEQKNEF KAAFDIFIQDAEDGCISTKE LGKVMRMLGQNPTPEELQEM IDEVDEDGSGTVDFDEFLVM MVRCMKDDS

Data sets:

assigned_chemical_shifts
- shift_set_1
- shift_set_2

Data type	Count
1H chemical shifts	1100
13C chemical shifts	292
15N chemical shifts	186

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	trout N-terminal domin of cardiac TnC	1
2	CALCIUM (II) ION	2

Entities:

Entity 1, trout N-terminal domin of cardiac TnC 89 residues - Formula weight is not available

1

MET

ASN

ASP

ILE

TYR

LYS

ALA

VAL

GLU

2

GLN

LEU

THR

ASP

GLU

GLN

LYS

ASN

GLU

PHE

3

LYS

ALA

PHE

ASP

ILE

PHE

ILE

GLN

ASP

4

ALA

GLU

ASP

GLY

CYS

ILE

SER

THR

LYS

GLU

5

LEU

GLY

LYS

VAL

MET

ARG

MET

LEU

GLY

GLN

6

ASN

PRO

THR

PRO

GLU

LEU

GLN

GLU

MET

7

ILE

ASP

GLU

VAL

ASP

GLU

ASP

GLY

SER

GLY

8

THR

VAL

ASP

PHE

ASP

GLU

PHE

LEU

VAL

MET

9

MET

VAL

ARG

CYS

MET

LYS

ASP

SER

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1

CA

Samples:

sample_1: trout troponin C, [U-90% 15N], 1.34 mM; imidazole 10 mM; KCl 100 mM; dithiothreitol 20 mM; NaN3 0.03%; 2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS) 0.2 mM

sample_2: trout troponin C, [U-96% 15N], 1.34 mM; imidazole 10 mM; KCl 100 mM; dithiothreitol 20 mM; NaN3 0.03%; 2,2-dimethyl-2-silapentane-5-sulfonic_acid_(DSS) 0.2 mM

conditions_1: pH: 6.9 na; temperature: 303 K

conditions_2: pH: 6.9 na; temperature: 280 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
15N DIPSI-HSQC	not available	not available	not available
15N NOESY-HSQC	not available	not available	not available
HNHA	not available	not available	not available
HNHB	not available	not available	not available
2D-NOESY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Varian INOVA 500 MHz
Varian Unity 600 MHz
Varian INOVA 800 MHz

PDB	1R2U 1R6P 4GJG
EMBL	CDQ82174
GB	AAP33791 ACQ58284
REF	NP_001117928 XP_003439031 XP_004546240 XP_005053154 XP_005448716