BMRB Entry 5974

Title:
Assignment of the 1H, 13C and 15N resonances of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei
Deposition date:
2003-10-16
Original release date:
2004-04-07
Authors:
Wu, Huan-Lin; Williams, Christopher; Upadhyay, Abhishek; Galyov, Edouard; Stefan, Bagby
Citation:

Citation: Wu, Huan-Lin; Williams, Christopher; Upadhyay, Abhishek; Galyov, Edouard; Bagby, Stefan. "Letter to the Editor: Assignment of the 1H, 13C and 15N resonances of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei "  J. Biomol. NMR 29, 215-216 (2004).
PubMed: 15014239

Assembly members:

Assembly members:
BopE, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Burkholderia pseudomallei   Taxonomy ID: 28450   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Burkholderia pseudomallei

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts910
13C chemical shifts672
15N chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BopE monomer1

Entities:

Entity 1, BopE monomer 187 residues - Formula weight is not available

1   GLYSERTHRGLYASPALALYSGLNALAILE
2   ARGHISPHEVALASPGLUALAVALLYSGLN
3   VALALAHISALAARGTHRPROGLUILEARG
4   GLNASPALAGLUPHEGLYARGGLNVALTYR
5   GLUALATHRLEUCYSALAILEPHESERGLU
6   ALALYSASPARGPHECYSMETASPPROALA
7   THRARGALAGLYASNVALARGPROALAPHE
8   ILEGLUALALEUGLYASPALAALAARGALA
9   THRGLYLEUPROGLYALAASPLYSGLNGLY
10   VALPHETHRPROSERGLYALAGLYTHRASN
11   PROLEUTYRTHRGLUILEARGLEUARGALA
12   ASPTHRLEUMETGLYALAGLULEUALAALA
13   ARGPROGLUTYRARGGLULEUGLNPROTYR
14   ALAARGGLNGLNALAILEASPLEUVALALA
15   ASNALALEUPROALAGLUARGSERASNTHR
16   LEUVALGLUPHEARGGLNTHRVALGLNTHR
17   LEUGLUALATHRTYRARGARGALAALAGLN
18   ASPALASERARGASPGLULYSGLYALATHR
19   ASNALAALAASPGLYALAALA

Samples:

sample_1: BopE, [U-95% 13C; U-90% 15N], 1.0 mM

Cond_1: pH: 5.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
All NMR experiments were standard Varian pulse sequences,sample_1not availableCond_1
or part of the Varian ProteinPack distribution, or weresample_1not availableCond_1
supplied by Prof Lewis Kay, Toronto.sample_1not availableCond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
EMBL CAH38998 CDU31564
GB AAU46021 ABA53377 ABN00329 ABN85504 ABN95087
REF WP_004188462 WP_004525325 WP_004528812 WP_004549546 WP_038730375
SP A2S1Q7 A3MCH6 A3NLC8 A3P6Z0 Q3JL30
AlphaFold A2S1Q7 A3MCH6 A3NLC8 A3P6Z0 Q3JL30

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks