BMRB Entry 5974

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5974

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Assignment of the 1H, 13C and 15N resonances of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei

Deposition date:

2003-10-16

Original release date:

2004-04-07

Authors:

Wu, Huan-Lin; Williams, Christopher; Upadhyay, Abhishek; Galyov, Edouard; Stefan, Bagby

Citation:

Citation: Wu, Huan-Lin; Williams, Christopher; Upadhyay, Abhishek; Galyov, Edouard; Bagby, Stefan. "Letter to the Editor: Assignment of the 1H, 13C and 15N resonances of the catalytic domain of guanine nucleotide exchange factor BopE from Burkholderia pseudomallei " J. Biomol. NMR 29, 215-216 (2004).
PubMed: 15014239

Assembly members:

Assembly members:
BopE, polymer, 187 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Burkholderia pseudomallei Taxonomy ID: 28450 Superkingdom: Bacteria Kingdom: not available Genus/species: Burkholderia pseudomallei

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
BopE: GSTGDAKQAIRHFVDEAVKQ VAHARTPEIRQDAEFGRQVY EATLCAIFSEAKDRFCMDPA TRAGNVRPAFIEALGDAARA TGLPGADKQGVFTPSGAGTN PLYTEIRLRADTLMGAELAA RPEYRELQPYARQQAIDLVA NALPAERSNTLVEFRQTVQT LEATYRRAAQDASRDEKGAT NAADGAA

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	910
13C chemical shifts	672
15N chemical shifts	175

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BopE monomer	1

Entities:

Entity 1, BopE monomer 187 residues - Formula weight is not available

1

GLY

SER

THR

GLY

ASP

ALA

LYS

GLN

ALA

ILE

2

ARG

HIS

PHE

VAL

ASP

GLU

ALA

VAL

LYS

GLN

3

VAL

ALA

HIS

ALA

ARG

THR

PRO

GLU

ILE

ARG

4

GLN

ASP

ALA

GLU

PHE

GLY

ARG

GLN

VAL

TYR

5

GLU

ALA

THR

LEU

CYS

ALA

ILE

PHE

SER

GLU

6

ALA

LYS

ASP

ARG

PHE

CYS

MET

ASP

PRO

ALA

7

THR

ARG

ALA

GLY

ASN

VAL

ARG

PRO

ALA

PHE

8

ILE

GLU

ALA

LEU

GLY

ASP

ALA

ARG

ALA

9

THR

GLY

LEU

PRO

GLY

ALA

ASP

LYS

GLN

GLY

10

VAL

PHE

THR

PRO

SER

GLY

ALA

GLY

THR

ASN

11

PRO

LEU

TYR

THR

GLU

ILE

ARG

LEU

ARG

ALA

12

ASP

THR

LEU

MET

GLY

ALA

GLU

LEU

ALA

13

ARG

PRO

GLU

TYR

ARG

GLU

LEU

GLN

PRO

TYR

14

ALA

ARG

GLN

ALA

ILE

ASP

LEU

VAL

ALA

15

ASN

ALA

LEU

PRO

ALA

GLU

ARG

SER

ASN

THR

16

LEU

VAL

GLU

PHE

ARG

GLN

THR

VAL

GLN

THR

17

LEU

GLU

ALA

THR

TYR

ARG

ALA

GLN

18

ASP

ALA

SER

ARG

ASP

GLU

LYS

GLY

ALA

THR

19

ASN

ALA

ASP

GLY

ALA

Samples:

sample_1: BopE, [U-95% 13C; U-90% 15N], 1.0 mM

Cond_1: pH: 5.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
All NMR experiments were standard Varian pulse sequences,	sample_1	not available	Cond_1
or part of the Varian ProteinPack distribution, or were	sample_1	not available	Cond_1
supplied by Prof Lewis Kay, Toronto.	sample_1	not available	Cond_1

Software:

No software information available

NMR spectrometers:

Varian INOVA 600 MHz

PDB	2JOK 2JOL
EMBL	CAH38998 CDU31564
GB	AAU46021 ABA53377 ABN00329 ABN85504 ABN95087
REF	WP_004188462 WP_004525325 WP_004528812 WP_004549546 WP_038730375
SP	A2S1Q7 A3MCH6 A3NLC8 A3P6Z0 Q3JL30
AlphaFold	A2S1Q7 A3MCH6 A3NLC8 A3P6Z0 Q3JL30