BMRB Entry 5970

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5970
MolProbity Validation Chart

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Title:
Solution Structure of Ribosomal Protein L18 from Bacillus stearothermophilus
Deposition date:
2003-10-14
Original release date:
2004-02-13
Authors:
Turner, C.; Moore, P.
Citation:

Citation: Turner, C.; Moore, P.. "The Solution Structure of Ribosomal Protein L18 from Bacillus stearothermophilus "  J. Mol. Biol. 335, 679-684 (2004).
PubMed: 14687565

Assembly members:

Assembly members:
Bacillus stearothermophius L18, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Geobacillus stearothermophilus   Taxonomy ID: 1422   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET13A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Bacillus stearothermophius L18: MITKVDRNAVRKKRHARIRK KIFGTTERPRLSVFRSNKHI YAQIIDDTKSATIVSASTLD KEFGLDSTNNIEAAKKVGEL VAKRALEKGIKQVVFDRGGY LYHGRVKALADAAREAGLEF

Data sets:
Data typeCount
1H chemical shifts597
13C chemical shifts245
15N chemical shifts114

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
150S Ribosomal Protein L181

Entities:

Entity 1, 50S Ribosomal Protein L18 120 residues - Formula weight is not available

1   METILETHRLYSVALASPARGASNALAVAL
2   ARGLYSLYSARGHISALAARGILEARGLYS
3   LYSILEPHEGLYTHRTHRGLUARGPROARG
4   LEUSERVALPHEARGSERASNLYSHISILE
5   TYRALAGLNILEILEASPASPTHRLYSSER
6   ALATHRILEVALSERALASERTHRLEUASP
7   LYSGLUPHEGLYLEUASPSERTHRASNASN
8   ILEGLUALAALALYSLYSVALGLYGLULEU
9   VALALALYSARGALALEUGLULYSGLYILE
10   LYSGLNVALVALPHEASPARGGLYGLYTYR
11   LEUTYRHISGLYARGVALLYSALALEUALA
12   ASPALAALAARGGLUALAGLYLEUGLUPHE

Samples:

sample_1: Bacillus stearothermophius L18, [U-15N], 1.5 mM; phosphate buffer 20 mM; NaCl 200 mM; MgCl2 5 mM; H2O 90%; D2O 10%

sample_2: Bacillus stearothermophius L18, [U-15N; U-13C], 1.5 mM; phosphate buffer 20 mM; NaCl 200 mM; MgCl2 5 mM; H2O 90%; D2O 10%

sample_3: Bacillus stearothermophius L18 1 mM; phosphate buffer 20 mM; NaCl 200 mM; MgCl2 5 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 6.0 na; temperature: 298 K; ionic strength: 225 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACOnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
15N TOCSY-HSQCnot availablenot availablenot available
15N NOESY-HSQCnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
13C NOESY-HSQCnot availablenot availablenot available

Software:

FELIX v97.0 - processing

Sparky v3.93 - data analysis

CNS v1.0 - structure solution, refinement

ARIA v1.2 - structure solution, refinement

NMR spectrometers:

  • Varian UNITY 600 MHz

Related Database Links:

PDB
DBJ BAD74407 GAD13888 GAJ56889
GB AAA22702 ACX76823 ADI25196 AEV17466 AGE20738
REF WP_011229635 WP_033008681 YP_003251305 YP_003669773 YP_004980566
SP P09415 Q5L3S3
AlphaFold P09415 Q5L3S3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks