BMRB Entry 5959

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5959

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions

Deposition date:

2003-09-29

Original release date:

2004-09-30

Authors:

Byeon, In-Ja; Li, Hongyuan; Tsai, Ming-Daw

Citation:

Citation: Li, Hongyuan; Byeon, In-Ja; Tsai, Ming-Daw. "Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions " J. Mol. Biol. 335, 371-381 (2004).
PubMed: 14659764

Assembly members:

Assembly members:
Antigen Ki-67, polymer, 128 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Antigen Ki-67: GSPEFPGGMWPTRRLVTIKR SGVDGPHFPLSLSTCLFGRG IECDIRIQLPVVSKQHCKIE IHEQEAILHNFSSTNPTQVN GSVIDEPVRLKHGDVITIID RSFRYENESLQNGRKSTEFP RKIREQEP

Data sets:

assigned_chemical_shifts
- ki67_FHA

Data type	Count
1H chemical shifts	692
13C chemical shifts	434
15N chemical shifts	103

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ki67 FHA monomer	1

Entities:

Entity 1, ki67 FHA monomer 128 residues - Formula weight is not available

1

GLY

SER

PRO

GLU

PHE

PRO

GLY

MET

TRP

2

PRO

THR

ARG

LEU

VAL

THR

ILE

LYS

ARG

3

SER

GLY

VAL

ASP

GLY

PRO

HIS

PHE

PRO

LEU

4

SER

LEU

SER

THR

CYS

LEU

PHE

GLY

ARG

GLY

5

ILE

GLU

CYS

ASP

ILE

ARG

ILE

GLN

LEU

PRO

6

VAL

SER

LYS

GLN

HIS

CYS

LYS

ILE

GLU

7

ILE

HIS

GLU

GLN

GLU

ALA

ILE

LEU

HIS

ASN

8

PHE

SER

THR

ASN

PRO

THR

GLN

VAL

ASN

9

GLY

SER

VAL

ILE

ASP

GLU

PRO

VAL

ARG

LEU

10

LYS

HIS

GLY

ASP

VAL

ILE

THR

ILE

ASP

11

ARG

SER

PHE

ARG

TYR

GLU

ASN

GLU

SER

LEU

12

GLN

ASN

GLY

ARG

LYS

SER

THR

GLU

PHE

PRO

13

ARG

LYS

ILE

ARG

GLU

GLN

GLU

PRO

Samples:

sample_1: Antigen Ki-67, [U-13C; U-15N], 0.3 – 0.5 mM; TrisHCl buffer 10 mM; DTT 2 mM; EDTA 1 mM

sample_2: Antigen Ki-670.3 – 0.5 mM; TrisHCl buffer 10 mM; DTT 2 mM; EDTA 1 mM

condition_1: pH: 8.4; temperature: 290 K; ionic strength: 0.01 M

condition_2: pH: 7.5; temperature: 290 K; ionic strength: 0.15 M

Experiments:

Name	Sample	Sample state	Sample conditions
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
15N-edited NOESY	not available	not available	not available
15N-edited TOCSY	not available	not available	not available
13C-edited NOESY	not available	not available	not available

Software:

XWINNMR v2.6 - processing

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DMX 600 MHz
Bruker DRX 600 MHz

PDB	1R21 2AFF
EMBL	CAA46519 CAA46520
GB	EAW49178 EAW49179
PIR	B48666
REF	NP_001139438 NP_002408 XP_002821326 XP_002821327 XP_004050323
SP	P46013
AlphaFold	P46013