BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5956

Title: NMR solution structure of Max in absence of DNA: Insight into the mechanism of DNA recognition by B-HLH-LZ transcription factors   PubMed: 15342239

Deposition date: 2003-09-19 Original release date: 2005-05-03

Authors: Sauve, Simon; Tremblay, Luc; Lavigne, Pierre

Citation: Sauve, S.; Tremblay, L.; Lavigne, P.. "The NMR solution structure of a mutant of the Max b/HLH/LZ free of DNA: insights into the specific and reversible DNA binding mechanism of dimeric transcription factors."  J. Mol. Biol. 342, 813-832 (2004).

Assembly members:
Max, polymer, 87 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pEt3a

Entity Sequences (FASTA):
Max: MADKRAHHNALERKRRDHIK DSFHSLRDSVPSLQGEKASR AQILDKATEYIQYMRRKVHT LQQDIDDLKRQNALLEQQVR ALEGSGC

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts75
1H chemical shifts386

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Max*VL subunit 11
2Max*VL subunit 21

Entities:

Entity 1, Max*VL subunit 1 87 residues - Formula weight is not available

1   METALAASPLYSARGALAHISHISASNALA
2   LEUGLUARGLYSARGARGASPHISILELYS
3   ASPSERPHEHISSERLEUARGASPSERVAL
4   PROSERLEUGLNGLYGLULYSALASERARG
5   ALAGLNILELEUASPLYSALATHRGLUTYR
6   ILEGLNTYRMETARGARGLYSVALHISTHR
7   LEUGLNGLNASPILEASPASPLEULYSARG
8   GLNASNALALEULEUGLUGLNGLNVALARG
9   ALALEUGLUGLYSERGLYCYS

Samples:

sample_1: Max, [U-15N], 30 mM

sample_2: Max, [U-13C; U-15N], 30 mM

Expt_condition_1: pH: 6.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
15N NOESY HSQCnot availablenot availablenot available
13C NOESY HSQCnot availablenot availablenot available
13C aromatic NOESY HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HCCH COSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian UNITY-INOVA 600 MHz

Related Database Links:

PDB

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts