BMRB Entry 5955

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5955

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Title:
Backbone and sidechain assignment of human VASP EVH2 domain
Deposition date:
2003-09-18
Original release date:
2003-12-19
Authors:
Zimmermann, Juergen; Oschkinat, Hartmut; Ball, Linda
Citation:

Citation: Zimmermann, Juergen; Labudde, Dirk; Jarchau, Thomas; Walter, Ulrich; Oschkinat, Hartmut; Ball, Linda. "Relaxation, equilibrium oligomerization and molecular symmetry of the VASP (336-380) EVH2 tetramer"  Biochemistry 41, 11143-11151 (2002).
PubMed: 12220179

Assembly members:

Assembly members:
Human VASP EVH2 domain, polymer, 47 residues, 5288 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Human VASP EVH2 domain: GSPSSSDYSDLQRVKQELLE EVKKELQKVKEEIIEAFVQE LRKRGSP

Data sets:
Data typeCount
13C chemical shifts148
15N chemical shifts49
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VASP EVH2 domain, chain 11
2VASP EVH2 domain, chain 21
3VASP EVH2 domain, chain 31
4VASP EVH2 domain, chain 41

Entities:

Entity 1, VASP EVH2 domain, chain 1 47 residues - 5288 Da.

1   GLYSERPROSERSERSERASPTYRSERASP
2   LEUGLNARGVALLYSGLNGLULEULEUGLU
3   GLUVALLYSLYSGLULEUGLNLYSVALLYS
4   GLUGLUILEILEGLUALAPHEVALGLNGLU
5   LEUARGLYSARGGLYSERPRO

Samples:

Sample_1: Human VASP EVH2 domain, [U-15N], 4.5 mM

Sample_2: Human VASP EVH2 domain, [U-15N; U-13C], 4.0 mM

Cond_1: pH: 6.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
15N-NOESY-HSQCnot availablenot availablenot available
13C-NOESY-HSQCnot availablenot availablenot available
13C-HMQC-NOESYnot availablenot availablenot available
CBCACONNHnot availablenot availablenot available
CBCANNHnot availablenot availablenot available
15N-HSQCnot availablenot availablenot available
15N-TOCSYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available

Software:

xwinnmr v2.6 - data collection, processing

ANSIG v3.3 - assignment, spectra handling

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAG37336 BAJ21098
EMBL CAA67147 CAA86523
GB AAH26019 AAH38224 AIC59345 EAW57362 EAW57363
REF NP_003361 XP_003817563 XP_005259256 XP_005259257 XP_008968979
SP P50552
AlphaFold P50552

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks